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Validation Data Gallery

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  • Western Blot (WB) analysis of A549 cells using CD155/PVR Polyclonal antibody (27486-1-AP)

    WB analysis of A549 using 27486-1-AP

    WB result of CD155/PVR antibody (27486-1-AP; 1:1000; room temperature for 1.5 hours) with wild-type and CD155/PVR knockout A549 cells.

  • Western Blot (WB) analysis of HT-1080 cells using CD155/PVR Polyclonal antibody (27486-1-AP)

    WB analysis of HT-1080 using 27486-1-AP

    HT-1080 cells were subjected to SDS PAGE followed by western blot with 27486-1-AP (CD155/PVR antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using Poliovirus receptor Polyclonal antibody (27486-1-AP)

    WB analysis of A549 using 27486-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 27486-1-AP (PVR antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HUVEC cells using Poliovirus receptor Polyclonal antibody (27486-1-AP)

    WB analysis of HUVEC using 27486-1-AP

    HUVEC cells were subjected to SDS PAGE followed by western blot with 27486-1-AP (Poliovirus receptor antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using CD155/PVR Polyclonal antibody (27486-1-AP)

    IHC staining of human cervical cancer using 27486-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 27486-1-AP (CD155/PVR antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using CD155/PVR Polyclonal antibody (27486-1-AP)

    IHC staining of human cervical cancer using 27486-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 27486-1-AP (CD155/PVR antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  • Featured Product
  • KD/KO Validated

CD155/PVR Polyclonal antibody

CD155/PVR Polyclonal Antibody for WB, IHC, ELISA
Cat No. 27486-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

PVR, Nectin like protein 5, NECL-5, NECL5, Necl 5

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHT-1080 cells, A549 cells, HUVEC cells
Positive IHC detected inhuman cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

27486-1-AP targets CD155/PVR in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CD155/PVR fusion protein Ag26657 相同性解析による交差性が予測される生物種
Full Name poliovirus receptor
Calculated molecular weight 45 kDa
Observed molecular weight 70 kDa
GenBank accession numberBC015542
Gene Symbol CD155/PVR
Gene ID (NCBI) 5817
RRIDAB_2880884
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP15151
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD155, also known as PVR, is a type I transmembrane glycoprotein in the immunoglobulin superfamily. It contains three extracellular immunoglobulin-like domains, D1-D3, of which D1 is recognized by the virus. Mature human CD155 consists of a 323 amino acid extracellular domain with one N-terminal V-type and two C2-type Ig-like domains, a 24 amino acid transmembrane segment, and a 50 amino acid cytoplasmic tail. CD155 is thought to play a role in adhesion by interaction with the ECM component vitronectin as well as a role in NK killing of tumor cells. CD155 binds to two receptors of NK cells, CD96 and CD226, and accumulates at cell-cell contact sites, leading to the formation of mature immune synapses between NK cells and target cells. CD155 serves as the entry receptor for poliovirus and thereby mediates human susceptibility to poliovirus infection.

Protocols

Product Specific Protocols
WB protocol for CD155/PVR antibody 27486-1-APDownload protocol
IHC protocol for CD155/PVR antibody 27486-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Nutr Biochem

Isothiocyanates attenuate immune checkpoint blockage therapy in gastric cancer via induction of PD-L1 expression

Authors - Qi Zhang
View Article