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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse lung tissue using PUS1 Polyclonal antibody (11512-1-AP)

    WB analysis of mouse lung using 11512-1-AP (same clone as 11512-1-PBS)

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 11512-1-AP (PUS1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11512-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using PUS1 Polyclonal antibody (11512-1-AP)

    WB analysis using 11512-1-AP (same clone as 11512-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 11512-1-AP (PUS1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11512-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using PUS1 Polyclonal antibody (11512-1-AP)

    WB analysis of HEK-293 using 11512-1-AP (same clone as 11512-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 11512-1-AP (PUS1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11512-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using PUS1 Polyclonal antibody (11512-1-AP)

    WB analysis of HeLa using 11512-1-AP (same clone as 11512-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 11512-1-AP (PUS1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11512-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using PUS1 Polyclonal antibody (11512-1-AP)

    IHC staining of human ovary tumor using 11512-1-AP (same clone as 11512-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 11512-1-AP (PUS1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11512-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using PUS1 Polyclonal antibody (11512-1-AP)

    IHC staining of human ovary tumor using 11512-1-AP (same clone as 11512-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 11512-1-AP (PUS1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11512-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using PUS1 Polyclonal antibody (11512-1-AP)

    IF Staining of MCF-7 using 11512-1-AP (same clone as 11512-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using PUS1 antibody (11512-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). This data was developed using the same antibody clone with 11512-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

PUS1 Polyclonal antibody, PBS Only

PUS1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 11512-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

EC:5.4.99.-, EC:5.4.99.12, MLASA, PP8985, pseudouridylate synthase 1

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

11512-1-PBS targets PUS1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag2071

Product name: Recombinant human PUS1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 128-427 aa of BC019320

Sequence: ENHGEDMRKMSFQRCARTDKGVSAAGQVVSLKVWLIDDILEKINSHLPSHIRILGLKRVTGGFNSKNRCDARTYCYLLPTFAFAHKDRDVQDETYRLSAETLQQVNRLLACYKGTHNFHNFTSQKGPQDPSACRYILEMYCEEPFVREGLEFAVIRVKGQSFMMHQIRKMVGLVVAIVKGYAPESVLERSWGTEKVDVPKAPGLGLVLERVHFEKYNQRFGNDGLHEPLDWAQEEGKVAAFKEEHIYPTIIGTERDERSMAQWLSTLPIHNFSATALTAGGTGAKVPSPLEGSEGDGDTD

相同性解析による交差性が予測される生物種
Full Name pseudouridylate synthase 1
Calculated molecular weight 427 aa, 47 kDa
Observed molecular weight 40 kDa, 44 kDa
GenBank accession numberBC019320
Gene Symbol PUS1
Gene ID (NCBI) 80324
RRIDAB_2284652
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y606
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

PUS1 is a protein-coding gene located on human chromosome 12q24.33, encoding a pseudouridine synthase that catalyzes the isomerization of uridine to pseudouridine (Ψ) in RNA molecules. This post-transcriptional modification is the most abundant RNA modification in cells and plays essential roles in RNA structure stabilization and function. (PMID: 38450158)

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