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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PURG Recombinant antibody (84399-3-RR)

    WB analysis using 84399-3-RR (same clone as 84399-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84399-3-RR (PURG antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84399-3-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PURG Recombinant antibody (84399-3-RR)

    IF Staining of HeLa using 84399-3-RR (same clone as 84399-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PURG antibody (84399-3-RR, Clone: 241504E7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84399-3-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84399-3-RR

    Affinity and Kinetic Characterization of 84399-3-RR

    Biolayer interferometry (BLl) kinetic assays of 84399-3-RR against Human PURG were performed. The affinity constant is 0.328 nM.

PURG Recombinant monoclonal antibody, PBS Only

PURG Uni-rAb® Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 84399-3-PBS
Clone No.241504E7

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

241504E7, PURG A, PURG B

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84399-3-PBS targets PURG in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag15820

Product name: Recombinant human PURG protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 125-182 aa of BC106708

Sequence: HYAHLGLKGHRQEHGHSKEQGSRRRQKHSAPSPPVSVGSEEHPHSVLKTDYIERDNRK

相同性解析による交差性が予測される生物種
Full Name purine-rich element binding protein G
Calculated molecular weight 347 aa, 40 kDa
Observed molecular weight40 kDa
GenBank accession numberBC106708
Gene Symbol PURG
Gene ID (NCBI) 29942
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purfication
UNIPROT IDQ9UJV8
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

PURG (Purine-rich element-binding protein G) is a member of the PUR protein family. It has 2 isoforms with a molecular weight of 37-40 kDa.

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