Validation Data Gallery
Tested Applications
| Positive WB detected in | A549 cells, HepG2 cells, Hela cells, U-87 MG cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 7 publications below |
| IHC | See 1 publications below |
| IF | See 1 publications below |
| IP | See 2 publications below |
| RIP | See 1 publications below |
Product Information
17733-1-AP targets PURA in WB, IHC, IF, IP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag12173 Product name: Recombinant human PURA protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-322 aa of BC036087 Sequence: MADRDSGSEQGGAALGSGGSLGHPGSGSGSGGGGGGGGGGGGSGGGGGGAPGGLQHETQELASKRVDIRNKRFYLDVKQNAKGRFLKIAEVGAGGNKSRLTLSMSVAVEFRDYLGDFIEHYAQLGPSQPPDLAQAQDEPRRALKSEFLVRENRKYYMDLKENQRGRFLRIRQTVNRGPGLGSTQGQTIALPAQGLIEFRDALAKLIDDYGVEEEPAELPEGTSLTVDNKRFFFDVGSNKYGVFMRVSEVKPTYRNSITVPYKVWAKFGHTFCKYSEEMKKIQEKQREKRAACEQLHQQQQQQQEETAAATLLLQGEEEGEED 相同性解析による交差性が予測される生物種 |
| Full Name | purine-rich element binding protein A |
| Calculated molecular weight | 322 aa, 35 kDa |
| Observed molecular weight | 35-40 kDa |
| GenBank accession number | BC036087 |
| Gene Symbol | PURA |
| Gene ID (NCBI) | 5813 |
| RRID | AB_2173875 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q00577 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
PURA, also named as Transcriptional activator protein Pur-alpha, is a 322 amino acid protein, which belongs to the PUR DNA-binding protein family and can form homodimer or heterodimer with PURB and heterotrimer with PURB and YBX1/Y-box protein 1. PURA is a probable transcription activator that specifically binds the purine-rich single strand of the PUR element located upstream of the MYC gene and may play a role in the initiation of DNA replication and in recombination.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for PURA antibody 17733-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nucleic Acids Res Corrigendum to article "Quantitative proteomics reveals that long non-coding RNA MALAT1 interacts with DBC1 to regulate p53 acetylation''. | ||
Cancer Res LncRNA AGPG confers endocrine resistance in breast cancer by promoting E2F1 activity | ||
EBioMedicine Genome-wide CRISPR/Cas9 screening identifies a targetable MEST-PURA interaction in cancer metastasis
| ||
Nucleic Acids Res Quantitative proteomics reveals that long non-coding RNA MALAT1 interacts with DBC1 to regulate p53 acetylation. | ||
Acta Histochem Protective effects of astaxanthin on subarachnoid hemorrhage-induced early brain injury: Reduction of cerebral vasospasm and improvement of neuron survival and mitochondrial function. |
