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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PUF60 Monoclonal antibody (68533-1-Ig)

    WB analysis using 68533-1-Ig (same clone as 68533-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68533-1-Ig (PUF60 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68533-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse testis tissue using PUF60 Monoclonal antibody (68533-1-Ig)

    WB analysis of mouse testis using 68533-1-Ig (same clone as 68533-1-PBS)

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 68533-1-Ig (PUF60 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68533-1-PBS in a different storage buffer formulation.

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PUF60 Monoclonal antibody, PBS Only

PUF60 Monoclonal Antibody for WB, Indirect ELISA
Cat No. 68533-1-PBS
Clone No.1A3A1

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, rabbit

Applications

WB, Indirect ELISA

Synonyms

FBP interacting repressor, FIR, FLJ31379, PUF60, Ro binding protein 1, RoBP1, RoBPI, Siah binding protein 1, Siah BP1, SIAHBP1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68533-1-PBS targets PUF60 in WB, Indirect ELISA applications and shows reactivity with Human, mouse, rat, rabbit samples.

Tested Reactivity Human, mouse, rat, rabbit
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PUF60 fusion protein Ag33930 相同性解析による交差性が予測される生物種
Full Name poly-U binding splicing factor 60KDa
Calculated molecular weight 60 kDa
Observed molecular weight60 kDa
GenBank accession numberBC008875
Gene Symbol PUF60
Gene ID (NCBI) 22827
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9UHX1
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Poly-U-binding factor 60(PUF60) is a splicing factor homologous to and complementary in function to U2AF, which involves in pre-mRNA splicing, a stepwise process initiated by binding. Also PUF60 involves in other nuclear processes such as apoptosis and transcription regulation. It can interact with and repress FUBP1-induced transcriptional activation via the core-TFIIH basal transcription factor. However, it has no repression activity in xeroderma pigmentosum complementation group B (XPB) cells.