Validation Data Gallery
Tested Applications
Recommended dilution
| Application | Dilution |
|---|---|
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Product Information
68533-1-PBS targets PUF60 in WB, Indirect ELISA applications and shows reactivity with Human, mouse, rat, rabbit samples.
| Tested Reactivity | Human, mouse, rat, rabbit |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag33930 Product name: Recombinant human PUF60 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 243-542 aa of BC008875 Sequence: DPTTGKHKGYGFIEYEKAQSSQDAVSSMNLFDLGGQYLRVGKAVTPPMPLLTPATPGGLPPAAAVAAAAATAKITAQEAVAGAAVLGTLGTPGLVSPALTLAQPLGTLPQAVMAAQAPGVITGVTPARPPIPVTIPSVGVVNPILASPPTLGLLEPKKEKEEEELFPESERPEMLSEQEHMSISGSSARHMVMQKLLRKQESTVMVLRNMVDPKDIDDDLEGEVTEECGKFGAVNRVIIYQEKQGEEEDAEIIVKIFVEFSIASETHKAIQALNGRWFAGRKVVAEVYDQERFDNSDLSA 相同性解析による交差性が予測される生物種 |
| Full Name | poly-U binding splicing factor 60KDa |
| Calculated molecular weight | 60 kDa |
| Observed molecular weight | 60 kDa |
| GenBank accession number | BC008875 |
| Gene Symbol | PUF60 |
| Gene ID (NCBI) | 22827 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q9UHX1 |
| Storage Buffer | PBS only{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -80°C. |
Background Information
Poly-U-binding factor 60(PUF60) is a splicing factor homologous to and complementary in function to U2AF, which involves in pre-mRNA splicing, a stepwise process initiated by binding. Also PUF60 involves in other nuclear processes such as apoptosis and transcription regulation. It can interact with and repress FUBP1-induced transcriptional activation via the core-TFIIH basal transcription factor. However, it has no repression activity in xeroderma pigmentosum complementation group B (XPB) cells.