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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using PUF60 Polyclonal antibody (10810-1-AP)

    WB analysis of HeLa using 10810-1-AP (same clone as 10810-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 10810-1-AP (PUF60 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10810-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse testis tissue using PUF60 Polyclonal antibody (10810-1-AP)

    WB analysis of mouse testis using 10810-1-AP (same clone as 10810-1-PBS)

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 10810-1-AP (PUF60 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10810-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using PUF60 Polyclonal antibody (10810-1-AP)

    WB analysis of HepG2 using 10810-1-AP (same clone as 10810-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 10810-1-AP (PUF60 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10810-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat testis tissue using PUF60 Polyclonal antibody (10810-1-AP)

    WB analysis of rat testis using 10810-1-AP (same clone as 10810-1-PBS)

    rat testis tissue were subjected to SDS PAGE followed by western blot with 10810-1-AP (PUF60 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10810-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using PUF60 Polyclonal antibody (10810-1-AP)

    IHC staining of human colon cancer using 10810-1-AP (same clone as 10810-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10810-1-AP (PUF60 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10810-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using PUF60 Polyclonal antibody (10810-1-AP)

    IHC staining of human breast cancer using 10810-1-AP (same clone as 10810-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10810-1-AP (PUF60 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10810-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using PUF60 Polyclonal antibody (10810-1-AP)

    IHC staining of human colon cancer using 10810-1-AP (same clone as 10810-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10810-1-AP (PUF60 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10810-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using PUF60 Polyclonal antibody (10810-1-AP)

    IHC staining of human breast cancer using 10810-1-AP (same clone as 10810-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10810-1-AP (PUF60 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10810-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

PUF60 Polyclonal antibody, PBS Only

PUF60 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 10810-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

10810-1-PBS targets PUF60 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PUF60 fusion protein Ag1053 相同性解析による交差性が予測される生物種
Full Name poly-U binding splicing factor 60KDa
Calculated molecular weight 60 kDa
Observed molecular weight 60 kDa
GenBank accession numberBC008875
Gene Symbol PUF60
Gene ID (NCBI) 22827
RRIDAB_2173583
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UHX1
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Poly-U-binding factor 60(PUF60) is a splicing factor homologous to and complementary in function to U2AF, which involves in pre-mRNA splicing, a stepwise process initiated by binding. Also PUF60 involves in other nuclear processes such as apoptosis and transcription regulation. It can interact with and repress FUBP1-induced transcriptional activation via the core-TFIIH basal transcription factor. However, it has no repression activity in xeroderma pigmentosum complementation group B (XPB) cells.