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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PUF-A,KIAA0020 Monoclonal antibody

PUF-A,KIAA0020 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1G4B7

Cat no : 67941-1-Ig

Synonyms

HLA HA8, KIAA0020, PEN, PUF6, PUF-A, PUM3, XTP5

Validation Data Gallery

Applications:
  • WB analysis using 67941-1-Ig

    WB analysis using 67941-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67941-1-Ig (KIAA0020 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • WB analysis of HSC-T6 using 67941-1-Ig

    WB analysis of HSC-T6 using 67941-1-Ig

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67941-1-Ig (KIAA0020 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of PC-12 using 67941-1-Ig

    WB analysis of PC-12 using 67941-1-Ig

    PC-12 cells were subjected to SDS PAGE followed by western blot with 67941-1-Ig (KIAA0020 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of NIH/3T3 using 67941-1-Ig

    WB analysis of NIH/3T3 using 67941-1-Ig

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67941-1-Ig (KIAA0020 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of 4T1 using 67941-1-Ig

    WB analysis of 4T1 using 67941-1-Ig

    4T1 cells were subjected to SDS PAGE followed by western blot with 67941-1-Ig (KIAA0020 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inHeLa cells, HSC-T6 cells, PC-12 cells, NIH/3T3 cells, 4T1 cells, HEK-293 cells, HepG2 cells, Jurkat cells, k-562 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:20000
Sample-dependent, check data in validation data gallery

Product Information

67941-1-Ig targets PUF-A,KIAA0020 in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PUF-A,KIAA0020 fusion protein Ag31324 相同性解析による交差性が予測される生物種
Full Name KIAA0020
Calculated molecular weight 648 aa, 74 kDa
Observed molecular weight68-70 kDa
GenBank accession numberBC016137
Gene symbol KIAA0020
Gene ID (NCBI) 9933
RRIDAB_2918693
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for PUF-A,KIAA0020 antibody 67941-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols