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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PTTG1IP Polyclonal antibody (12575-1-AP)

    WB analysis using 12575-1-AP (same clone as 12575-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 12575-1-AP (PTTG1IP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12575-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HEK-293 cells using PTTG1IP Polyclonal antibody (12575-1-AP)

    IP experiment of HEK-293 using 12575-1-AP (same clone as 12575-1-PBS)

    IP result of anti-PTTG1IP (IP:12575-1-AP, 4ug; Detection:12575-1-AP 1:300) with HEK-293 cells lysate 2600 ug. This data was developed using the same antibody clone with 12575-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using PTTG1IP Polyclonal antibody (12575-1-AP)

    IHC staining of human testis using 12575-1-AP (same clone as 12575-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis using 12575-1-AP (PTTG1IP antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 12575-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using PTTG1IP Polyclonal antibody (12575-1-AP)

    IHC staining of human testis using 12575-1-AP (same clone as 12575-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis using 12575-1-AP (PTTG1IP antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 12575-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using PTTG1IP Polyclonal antibody (12575-1-AP)

    IHC staining of human liver using 12575-1-AP (same clone as 12575-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver using 12575-1-AP (PTTG1IP antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 12575-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using PTTG1IP Polyclonal antibody (12575-1-AP)

    IHC staining of human liver using 12575-1-AP (same clone as 12575-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver using 12575-1-AP (PTTG1IP antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 12575-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using PTTG1IP Polyclonal antibody (12575-1-AP)

    IHC staining of human colon using 12575-1-AP (same clone as 12575-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon using 12575-1-AP (PTTG1IP antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 12575-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using PTTG1IP Polyclonal antibody (12575-1-AP)

    IHC staining of human colon using 12575-1-AP (same clone as 12575-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon using 12575-1-AP (PTTG1IP antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 12575-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PTTG1IP Polyclonal antibody (12575-1-AP)

    IF Staining of HeLa using 12575-1-AP (same clone as 12575-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PTTG1IP antibody (12575-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 12575-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of PC-3 cells using PTTG1IP Polyclonal antibody (12575-1-AP)

    IF Staining of PC-3 using 12575-1-AP (same clone as 12575-1-PBS)

    Immunofluorescent analysis of PC-3 cells using 12575-1-AP (PTTG1IP antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12575-1-PBS in a different storage buffer formulation.

PTTG1IP Polyclonal antibody, PBS Only

PTTG1IP Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 12575-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

12575-1-PBS targets PTTG1IP in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag3250

Product name: Recombinant human PTTG1IP protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-180 aa of BC012858

Sequence: MAPGVARGPTPYWRLRLGGAALLLLLIPVAAAQEPPGAACSQNTNKTCEECLKNVSCLWCNTNKACLDYPVTSVLPPASLCKLSSARWGVCWVNFEALIITMSVVGGTLLLGIAICCCCCCRRKRSRKPDRSEEKAMREREERRIRQEERRAEMKTRHDEIRKKYGLFKEENPYARFENN

相同性解析による交差性が予測される生物種
Full Name pituitary tumor-transforming 1 interacting protein
Calculated molecular weight 180 aa, 20 kDa
Observed molecular weight 26 kDa
GenBank accession numberBC012858
Gene Symbol PTTG1IP
Gene ID (NCBI) 754
RRIDAB_10644145
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP53801
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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