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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Securin Recombinant antibody (83981-4-RR)

    IF Staining of HepG2 using 83981-4-RR (same clone as 83981-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Securin antibody (83981-4-RR, Clone: 241090G10 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83981-4-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Securin Recombinant antibody (83981-4-RR)

    IF Staining of HepG2 using 83981-4-RR (same clone as 83981-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Securin antibody (83981-4-RR, Clone: 241090G10 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83981-4-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of Jurkat cells using Securin Recombinant antibody (83981-4-RR)

    FC experiment of Jurkat using 83981-4-RR (same clone as 83981-4-PBS)

    1x10^6 Jurkat cells were intracellularly stained with 0.25 ug Securin Recombinant antibody (83981-4-RR, Clone:241090G10) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83981-4-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of A431 cells using Securin Recombinant antibody (83981-4-RR)

    FC experiment of A431 using 83981-4-RR (same clone as 83981-4-PBS)

    1x10^6 A431 cells were intracellularly stained with 0.25 ug Securin Recombinant antibody (83981-4-RR, Clone:241090G10) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83981-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83981-4-RR

    Affinity and Kinetic Characterization of 83981-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83981-4-RR against Human Securin were performed. The affinity constant is 0.155 nM.

Securin Recombinant monoclonal antibody, PBS Only

Securin Uni-rAb® Recombinant Antibody for IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA
Cat No. 83981-4-PBS
Clone No.241090G10

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Synonyms

PTTG1, 241090G10, EAP1, Esp1-associated protein, hPTTG

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83981-4-PBS targets Securin in IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag12670

Product name: Recombinant human PTTG1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-202 aa of BC101834

Sequence: MATLIYVDKENGEPGTRVVAKDGLKLGSGPSIKALDGRSQVSTPRFGKTFDAPPALPKATRKALGTVNRATEKSVKTKGPLKQKQPSFSAKKMTEKTVKAKSSVPASDDAYPEIEKFFPFNPLDFESFDLPEEHQIAHLPLSGVPLMILDEERELEKLFQLGPPSPVKMPSPPWESNLLQSPSSILSTLDVELPPVCCDIDI

相同性解析による交差性が予測される生物種
Full Name pituitary tumor-transforming 1
Calculated molecular weight 202 aa, 22 kDa
GenBank accession numberBC101834
Gene Symbol Securin
Gene ID (NCBI) 9232
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDO95997
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Securin, also known as the pituitary tumor transforming gene (PTTG1), was first isolated from rat pituitary tumor cells. Securin is a multi-functional protein that regulates sister chromatin segregation in mitosis, DNA repair, gene transcription, metabolism, and organ development. Securin is normally expressed in tissues with high proliferative activity, including the spleen, thymus gland, and testis, whereas it is rarely expressed in differentiated mature tissues (PMID: 22563433, PMID: 36123907). The calculated molecular weight of Securin is 22 kDa. With post-translational modification, the molecular weight of Securin will be migrated to 28 kDa.