Validation Data Gallery
Tested Applications
| Positive WB detected in | Daudi cells, HepG2 cells, MDA-MB-231 cells |
| Positive IHC detected in | human urothelial carcinoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | U2OS cells, HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 3 publications below |
| WB | See 14 publications below |
| IHC | See 6 publications below |
| IF | See 7 publications below |
| IP | See 1 publications below |
Product Information
18040-1-AP targets Securin in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with Human samples.
| Tested Reactivity | Human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag12670 Product name: Recombinant human PTTG1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-202 aa of BC101834 Sequence: MATLIYVDKENGEPGTRVVAKDGLKLGSGPSIKALDGRSQVSTPRFGKTFDAPPALPKATRKALGTVNRATEKSVKTKGPLKQKQPSFSAKKMTEKTVKAKSSVPASDDAYPEIEKFFPFNPLDFESFDLPEEHQIAHLPLSGVPLMILDEERELEKLFQLGPPSPVKMPSPPWESNLLQSPSSILSTLDVELPPVCCDIDI 相同性解析による交差性が予測される生物種 |
| Full Name | pituitary tumor-transforming 1 |
| Calculated molecular weight | 202 aa, 22 kDa |
| Observed molecular weight | 28 kDa |
| GenBank accession number | BC101834 |
| Gene Symbol | Securin |
| Gene ID (NCBI) | 9232 |
| RRID | AB_2173410 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O95997 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Securin, also known as the pituitary tumor transforming gene (PTTG1), was first isolated from rat pituitary tumor cells. Securin is a multi-functional protein that regulates sister chromatin segregation in mitosis, DNA repair, gene transcription, metabolism, and organ development. Securin is normally expressed in tissues with high proliferative activity, including the spleen, thymus gland, and testis, whereas it is rarely expressed in differentiated mature tissues (PMID: 22563433, PMID: 36123907). The calculated molecular weight of Securin is 22 kDa. With post-translational modification, the molecular weight of Securin will be migrated to 28 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for Securin antibody 18040-1-AP | Download protocol |
| IHC protocol for Securin antibody 18040-1-AP | Download protocol |
| WB protocol for Securin antibody 18040-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Adv Res Tankyrase activity is essential for asymmetric division and chromosome segregation in oocyte meiosis | ||
Adv Sci (Weinh) The Hypoxia-Associated High-Risk Cell Subpopulation Distinctly Enhances the Progression of Glioma | ||
Cancer Lett UBE2C promotes pancreatic tumorigenesis by KRAS stabilization via APC/CCDH1-mediated WDR76 degradation | ||
J Environ Manage Integrative network and computational toxicology reveal the molecular mechanisms in PFOA-induced spermatogenic disorder | ||
Oncogene Phosphorylated PTTG1 switches its subcellular distribution and promotes β-catenin stabilization and subsequent transcription activity
| ||
Curr Biol Securin acetylation prevents precocious separase activation and premature sister chromatid separation |