Validation Data Gallery
Tested Applications
| Positive WB detected in | COLO 320 cells, Caco-2 cells, HeLa cells, Jurkat cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
17651-1-AP targets PTRH1 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag11893 Product name: Recombinant human PTRH1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-214 aa of BC047012 Sequence: MRPGGFLGAGQRLSRAMSRCVLEPRPPGKRWMVAGLGNPGLPGTRHSVGMAVLGQLARRLGVAESWTRDRHCAADLALAPLGDAQLVLLRPRRLMNANGRSVARAAELFGLTAEEVYLVHDELDKPLGRLALKLGGSARGHNGVRSCISCLNSNAMPRLRVGIGRPAHPEAVQAHVLGCFSPAEQELLPLLLDRATDLILDHIRERSQGPSLGP 相同性解析による交差性が予測される生物種 |
| Full Name | peptidyl-tRNA hydrolase 1 homolog (S. cerevisiae) |
| Calculated molecular weight | 214 aa, 23 kDa |
| Observed molecular weight | 23 kDa |
| GenBank accession number | BC047012 |
| Gene Symbol | PTRH1 |
| Gene ID (NCBI) | 138428 |
| RRID | AB_3741927 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q86Y79 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Peptidyl-tRNA hydrolase 1 homolog(PTRH1), also known as PTH1, is peptidyl-tRNA hydrolase that cleaves nascent chains-tRNAs that are not stably fixed in the P-site of 60S ribosome-nascent chain complexes. PTRH1 functions in the ribosome-associated quality control (RQC) pathway and is involved in the degradation of nonubiquitinated nascent chain (NC) polypeptides arising from interrupted translation(PMID:30244831).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for PTRH1 antibody 17651-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |