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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PTPRR Polyclonal antibody (17937-1-AP)

    WB analysis using 17937-1-AP (same clone as 17937-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 17937-1-AP (PTPRR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17937-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using PTPRR Polyclonal antibody (17937-1-AP)

    WB analysis of HeLa using 17937-1-AP (same clone as 17937-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 17937-1-AP (PTPRR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17937-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse brain tissue using PTPRR Polyclonal antibody (17937-1-AP)

    WB analysis of mouse brain using 17937-1-AP (same clone as 17937-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 17937-1-AP (PTPRR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17937-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of COLO 320 cells using PTPRR Polyclonal antibody (17937-1-AP)

    WB analysis of COLO 320 using 17937-1-AP (same clone as 17937-1-PBS)

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 17937-1-AP (PTPRR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17937-1-PBS in a different storage buffer formulation.

PTPRR Polyclonal antibody, PBS Only

PTPRR Polyclonal Antibody for WB, ELISA
Cat No. 17937-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

Ch-1PTPase, EC PTP, EC:3.1.3.48, ECPTP, NC-PTPCOM1

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

17937-1-PBS targets PTPRR in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag12145

Product name: Recombinant human PTPRR protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-412 aa of BC110900

Sequence: MILYRLKERFQLSLRQDKEKNQEIHLSPITLQPALSEAKTVHSMVQPEQAPKVLNVVVDPQGRGAPEIRATTATSVCPSPFKMKPIGLQERRGSNVSLTLDMSSLGNIEPFVSIPTPREKVAMEYLQSASRILTRSQLRDVVASSHLLQSEFMEIPMNFVDPKEIDIPRHGTKNRYKTILPNPLSRVCLRPKNVTDSLSTYINANYIRGYSGKEKAFIATQGPMINTVDDFWQMVWQEDSPVIVMITKLKEKNEKCVLYWPEKRGIYGKVEVLVISVNECDNYTIRNLVLKQGSHTQHVKHYWYTSWPDHKTPDSAQPLLQLMLDVEEDRLASQGRGPVVVHCSAGIGRTGCFIATSIGCQQLKEEGVVDALSIVCQLRMDRGGMVQTSEQYEFVHHALCLYESRLSAETVQ

相同性解析による交差性が予測される生物種
Full Name protein tyrosine phosphatase, receptor type, R
Calculated molecular weight657 aa, 74 kDa
Observed molecular weight 32 kDa, 47 kDa, 65 kDa
GenBank accession numberBC110900
Gene Symbol PTPRR
Gene ID (NCBI) 5801
RRIDAB_10694430
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ15256
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

PTPRR(protein tyrosine phosphatase, receptor type, R) is also named as PCPTP1, PTP-SL, PTPBR7 and belongs to the protein-tyrosine phosphatase family. It sequesters mitogen-activated protein kinases (MAPKs) such as MAPK1, MAPK3 and MAPK14 in the cytoplasm in an inactive form. It has four isoforms that they have molecular weights of 72, 60, 42 and 37 kDa, respectively, and differ in the length of their N-terminal part which result in distinct subcellular localizations. All PTPRR isoforms are found to be short-lived proteins, with half-lives of around 3-5 hours, and are constitutively phosphorylated on one or two of the previously identified protein kinase A and MAP kinase target sites.

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