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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite®594-conjugated PTPRO Monoclonal antibody

PTPRO Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Pig

Applications

IF

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

2F2B4

Cat no : CL594-67000

Synonyms

GLEPP1, PTP U2, PTPase U2, PTPRO, PTPU2, R PTP O

Validation Data Gallery

Applications:
  • IF Staining of human kidney using CL594-67000

    IF Staining of human kidney using CL594-67000

    Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using CoraLite®594 PTPRO antibody (CL594-67000, Clone: 2F2B4 ) at dilution of 1:100.

Tested Applications

Positive IF detected inhuman kidney tissue
For other applications, we recommend the unconjugated version of this antibody, 67000-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

CL594-67000 targets PTPRO in IF applications and shows reactivity with Human, Pig samples.

Tested Reactivity Human, Pig
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen PTPRO fusion protein Ag8284 相同性解析による交差性が予測される生物種
Full Name protein tyrosine phosphatase, receptor type, O
Calculated molecular weight 138 kDa
Observed molecular weight 160 kDa, 180-220 kDa
GenBank accession numberBC035960
Gene symbol PTPRO
Gene ID (NCBI) 5800
RRIDAB_2920069
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage.

Background Information

PTPRO(receptor-type tyrosine-protein phosphatase O) is also named as GLEPP1, PTPU2 and belongs to the protein-tyrosine phosphatase family. The protein is a receptor-like membrane protein tyrosine phosphatase expressed at the apical membrane of the podocyte foot processes in the kidney(PMID:21722858). The 1,159-amino acid predicted mature protein contains a large extracellular domain, a single transmembrane domain, and a single intracellular PTPase domain. Defects in PTPRO are the cause of nephrotic syndrome type 6 (NPHS6). In reducing conditions, PTPRO can form a smear from 180 to 220 kDa; In non-reducing conditions, a band appeares around 350 kDa, which likely represents the dimeric form of full-length PTPRO (PMID: 19573017).

Protocols

Product Specific Protocols
IF protocol for CL594 PTPRO antibody CL594-67000Download protocol
Standard Protocols
Click here to view our Standard Protocols