Validation Data Gallery
Tested Applications
| Positive WB detected in | MCF-7 cells, MDA-MB-231 cells, U-87 MG cells, rat eye tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
30763-1-AP targets PTPRM/RPTPmu in WB, ELISA applications and shows reactivity with human, rat samples.
| Tested Reactivity | human, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag33475 Product name: Recombinant human PTPRM protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 765-880 aa of NM_002845 Sequence: KKRKLAKKRKETMSSTRQEMTVMVNSMDKSYAEQGTNCDEAFSFMDTHNLNGRSVSSPSSFTMKTNTLSTSVPNSYYPDETHTMASDTSSLVQSHTYKKREPADVPYQTGQLHPAI 相同性解析による交差性が予測される生物種 |
| Full Name | protein tyrosine phosphatase, receptor type, M |
| Calculated molecular weight | 164KD |
| Observed molecular weight | 80 kDa, 100 kDa, 200 kDa |
| GenBank accession number | NM_002845 |
| Gene Symbol | PTPRM |
| Gene ID (NCBI) | 5797 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P28827 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
PTPRM, also known as protein tyrosine phosphatase receptor type M or RPTPmu, is a protein with a molecular weight of approximately 155 kDa. It is a receptor-type tyrosine phosphatase involved in cell-cell adhesion, migration, and signaling. It exists in two forms, either a full-length 200 kDa form or as two noncovalently associated fragments of 100 kDa each.Additional unidentified serine proteases cleave PTPμ in GBM cells to yield smaller extracellular fragments with MWs of 78 and 55 kDa (PMID: 25223585).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for PTPRM/RPTPmu antibody 30763-1-AP | Download protocol |
| WB protocol for PTPRM/RPTPmu antibody 30763-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |