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Validation Data Gallery

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  • Western Blot (WB) analysis of multi-cells/tissue using PTPRJ Polyclonal antibody (55123-1-AP)

    WB analysis of multi-cells/tissue using 55123-1-AP (same clone as 55123-1-PBS)

    WB result of 55123-1-AP. This data was developed using the same antibody clone with 55123-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of K-562 cells using PTPRJ Polyclonal antibody (55123-1-AP)

    WB analysis of K-562 using 55123-1-AP (same clone as 55123-1-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 55123-1-AP (PTPRJ antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55123-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using PTPRJ Polyclonal antibody (55123-1-AP)

    WB analysis using 55123-1-AP (same clone as 55123-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 55123-1-AP (PTPRJ antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55123-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using PTPRJ Polyclonal antibody (55123-1-AP)

    WB analysis of HeLa using 55123-1-AP (same clone as 55123-1-PBS)

    WB result of PTPRJ antibody (55123-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-PTPRJ transfected HeLa cells. This data was developed using the same antibody clone with 55123-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using PTPRJ Polyclonal antibody (55123-1-AP)

    WB analysis of Jurkat using 55123-1-AP (same clone as 55123-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 55123-1-AP (PTPRJ antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55123-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using PTPRJ Polyclonal antibody (55123-1-AP)

    IHC staining of human tonsillitis using 55123-1-AP (same clone as 55123-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 55123-1-AP (PTPRJ Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55123-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using PTPRJ Polyclonal antibody (55123-1-AP)

    IHC staining of human tonsillitis using 55123-1-AP (same clone as 55123-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 55123-1-AP (PTPRJ Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55123-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

PTPRJ Polyclonal antibody, PBS Only

PTPRJ Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 55123-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

DEP-1, DEP1, DEP 1, Density-enhanced phosphatase 1, Density enhanced phosphatase 1

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

55123-1-PBS targets PTPRJ in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

Peptide

相同性解析による交差性が予測される生物種
Full Name protein tyrosine phosphatase, receptor type, J
Calculated molecular weight 146 kDa
Observed molecular weight 146-170 kDa
GenBank accession numberNM_002843
Gene Symbol PTPRJ
Gene ID (NCBI) 5795
RRIDAB_10859259
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ12913
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

PTPRJ, also named as CD148 and DEP1, belongs to the protein-tyrosine phosphatase family and Receptor class 3 subfamily. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. PTPRJ may contribute to the mechanism of contact inhibition of cell growth. PTPRJ is present in all hematopoietic lineages, and was shown to negatively regulate T cell receptor signaling possibly through interfering with the phosphorylation of Phospholipase C Gamma 1 (PLCG1) and Linker for Activation of T Cells (LAT). It was also found to dephosphorylate PDGF beta receptor, and may be involved in UV-induced signal transduction. The antibody is specific to PTPRJ.

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