Validation Data Gallery
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Tested Applications
| Positive WB detected in | HeLa cells, HSC-T6 cells, 4T1 cells, HEK293 cells, A549 cells, LNCaP cells, MCF-7 cells, U2OS cells, Jurkat cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
68070-1-Ig targets PTPRF in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.
| Tested Reactivity | Human, mouse, rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag25441 Product name: Recombinant human PTPRF protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1080-1150 aa of BC048768 Sequence: HLVSIRTAPDLLPHKPLPASAYIEDGRFDLSMPHVQDPSLVRWFYIVVVPIDRVGGSMLTPRWSTPEELEL 相同性解析による交差性が予測される生物種 |
| Full Name | protein tyrosine phosphatase, receptor type, F |
| Calculated molecular weight | 1898 aa, 212 kDa |
| Observed molecular weight | 150 kDa, 213 kDa |
| GenBank accession number | BC048768 |
| Gene Symbol | PTPRF |
| Gene ID (NCBI) | 5792 |
| RRID | AB_2918811 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P10586 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for PTPRF antibody 68070-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |