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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PTPRCAP Polyclonal antibody

PTPRCAP Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 28286-1-AP

Synonyms

CD45 AP, CD45 associated protein, LPAP, PTPRC associated protein, PTPRCAP

Validation Data Gallery

Applications:
  • WB analysis using 28286-1-AP

    WB analysis using 28286-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28286-1-AP (PTPRCAP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of Raji using 28286-1-AP

    WB analysis of Raji using 28286-1-AP

    Raji cells were subjected to SDS PAGE followed by western blot with 28286-1-AP (PTPRCAP antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • WB analysis of Jurkat using 28286-1-AP

    WB analysis of Jurkat using 28286-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 28286-1-AP (PTPRCAP antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • WB analysis of K-562 using 28286-1-AP

    WB analysis of K-562 using 28286-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 28286-1-AP (PTPRCAP antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human spleen using 28286-1-AP

    IHC staining of human spleen using 28286-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 28286-1-AP (PTPRCAP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human spleen using 28286-1-AP

    IHC staining of human spleen using 28286-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 28286-1-AP (PTPRCAP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human tonsillitis using 28286-1-AP

    IHC staining of human tonsillitis using 28286-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 28286-1-AP (PTPRCAP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human tonsillitis using 28286-1-AP

    IHC staining of human tonsillitis using 28286-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 28286-1-AP (PTPRCAP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inJurkat cells, K-562 cells, Raji cells
Positive IHC detected inhuman spleen tissue, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

28286-1-AP targets PTPRCAP in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PTPRCAP fusion protein Ag27236 相同性解析による交差性が予測される生物種
Full Name protein tyrosine phosphatase, receptor type, C-associated protein
Calculated molecular weight 206 aa, 21 kDa
Observed molecular weight 32 kDa
GenBank accession numberBC113859
Gene symbol PTPRCAP
Gene ID (NCBI) 5790
RRIDAB_2881105
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for PTPRCAP antibody 28286-1-APDownload protocol
IHC protocol for PTPRCAP antibody 28286-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Discov Oncol

New immunological potential markers for triple negative breast cancer: IL18R1, CD53, TRIM, Jaw1, LTB, PTPRCAP.

Authors - Paolo Marchetti
View Article