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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PTPN7 Polyclonal antibody (15286-1-AP)

    WB analysis using 15286-1-AP (same clone as 15286-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 15286-1-AP (PTPN7 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15286-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of K-562 cells using PTPN7 Polyclonal antibody (15286-1-AP)

    WB analysis of K-562 using 15286-1-AP (same clone as 15286-1-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 15286-1-AP (PTPN7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15286-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of K-562 cells using PTPN7 Polyclonal antibody (15286-1-AP)

    WB analysis of K-562 using 15286-1-AP (same clone as 15286-1-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 15286-1-AP (PTPN7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15286-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Raji cells using PTPN7 Polyclonal antibody (15286-1-AP)

    WB analysis of Raji using 15286-1-AP

    Raji cells were subjected to SDS PAGE followed by western blot with 15286-1-AP (PTPN7 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using PTPN7 Polyclonal antibody (15286-1-AP)

    IHC staining of human tonsillitis using 15286-1-AP (same clone as 15286-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 15286-1-AP (PTPN7 antibody) at dilution of 1:400 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15286-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using PTPN7 Polyclonal antibody (15286-1-AP)

    IHC staining of human tonsillitis using 15286-1-AP (same clone as 15286-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 15286-1-AP (PTPN7 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15286-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using PTPN7 Polyclonal antibody (15286-1-AP)

    IHC staining of human tonsillitis using 15286-1-AP (same clone as 15286-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 15286-1-AP (PTPN7 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15286-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using PTPN7 Polyclonal antibody (15286-1-AP)

    IHC staining of human lymphoma using 15286-1-AP (same clone as 15286-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 15286-1-AP (PTPN7 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15286-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using PTPN7 Polyclonal antibody (15286-1-AP)

    IHC staining of human lymphoma using 15286-1-AP (same clone as 15286-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 15286-1-AP (PTPN7 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15286-1-PBS in a different storage buffer formulation.

PTPN7 Polyclonal antibody, PBS Only

PTPN7 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 15286-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

15286-1-PBS targets PTPN7 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag7453

Product name: Recombinant human PTPN7 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-399 aa of BC001746

Sequence: MGASFWPIRQAREQQRRALSFRQTSWLSEPPLGPAPHLSMVQAHGGRSRAQPLTLSLGAAMTQPPPEKTPAKKHVRLQERRGSNVALMLDVRSLGAVEPICSVNTPREVTLHFLRTAGHPLTRWALQRQPPSPKQLEEEFLKIPSNFVSPEDLDIPGHASKDRYKTILPNPQSRVCLGRAQSQEDGDYINANYIRGYDGKEKVYIATQGPMPNTVSDFWEMVWQEEVSLIVMLTQLREGKEKCVHYWPTEEETYGPFQIRIQDMKECPEYTVRQLTIQYQEERRSVKHILFSAWPDHQTPESAGPLLRLVAEVEESPETAAHPGPIVVHCSAGIGRTGCFIATRIGCQQLKARGEVDILGIVCQLRLDRGGMIQTAEQYQFLHHTLALYAGQLPEEPSP

相同性解析による交差性が予測される生物種
Full Name protein tyrosine phosphatase, non-receptor type 7
Calculated molecular weight 41 kDa
Observed molecular weight 38-40 kDa
GenBank accession numberBC001746
Gene Symbol PTPN7
Gene ID (NCBI) 5778
RRIDAB_2173831
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP35236
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

PTPN7, also known as haematopoietic PTP (HePTP), belongs to the R7 family of protein tyrosine phosphatases (PTPs) which all contain a conserved 16 amino acid sequence at their N-termini (PMID: 9857190). PTPN7, via negative regulation of MAPK signalling, plays a key role in T-cell and B-cell development and is the only known PTP whose expression in T-lymphocytes is induced by IL-2 (PMID: 15980098). PTPN7 is a 38 kDa non-receptor PTP expressed predominately in leukocytes, spleen and thymus (PMID: 1530918, 24265715, 19636879).

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