Validation Data Gallery
Tested Applications
| Positive WB detected in | A375 cells, HeLa cells, HepG2 cells |
| Positive IF/ICC detected in | Starvation treated hTERT-RPE1 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
87561-1-RR targets PTPN23 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag41272 Product name: Recombinant human PTPN23 protein Source: e coli.-derived, PET30a Tag: 6*His Domain: 420-619 aa of BC004881 Sequence: QEKLHLRFCYEAVVRHVEQVLQRHGVPPPCKPLASASISQKNHLPQDSQDLVLGGDVPISSIQATIAKLSIRPPGGLESPVASLPGPAEPPGLPPASLPESTPIPSSSPPPLSSPLPEAPQPKEEPPVPEAPSSGPPSSSLELLASLTPEAFSLDSSLRGKQRMSKHNFLQAHNGQGLRATRPSDDPLSLLDPLWTLNKT 相同性解析による交差性が予測される生物種 |
| Full Name | protein tyrosine phosphatase, non-receptor type 23 |
| Calculated molecular weight | 179 kDa |
| Observed molecular weight | 179-200 kDa |
| GenBank accession number | BC004881 |
| Gene Symbol | PTPN23 |
| Gene ID (NCBI) | 25930 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q9H3S7 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
PTPN23 (Protein Tyrosine Phosphatase Non-receptor Type 23), also known as HD-PTP, is a member of the non-receptor class of protein tyrosine phosphatases that plays a critical role in endosomal trafficking and receptor downregulation. Structurally unique due to its extended C-terminal region containing a Bro1 domain, PTPN23 functions as a core component of the ESCPE-1 (Endosomal SNX-BAR Sorting Complex for Promoting Exit-1) complex and regulates the dephosphorylation of key endosomal proteins, thereby controlling cargo sorting, multivesicular body (MVB) biogenesis, and lysosomal degradation of cell surface receptors such as EGFR. Beyond its role in endocytosis, PTPN23 is involved in ciliogenesis, cell migration, and cytokinesis.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for PTPN23 antibody 87561-1-RR | Download protocol |
| WB protocol for PTPN23 antibody 87561-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |