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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PTPN23 Polyclonal antibody (10472-1-AP)

    WB analysis using 10472-1-AP (same clone as 10472-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 10472-1-AP (PTPN23 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10472-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using PTPN23 Polyclonal antibody (10472-1-AP)

    WB analysis of HeLa using 10472-1-AP (same clone as 10472-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 10472-1-AP (PTPN23 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10472-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using PTPN23 Polyclonal antibody (10472-1-AP)

    WB analysis of HeLa using 10472-1-AP (same clone as 10472-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 10472-1-AP (PTPN23 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10472-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A375 cells using PTPN23 Polyclonal antibody (10472-1-AP)

    WB analysis of A375 using 10472-1-AP (same clone as 10472-1-PBS)

    A375 cells were subjected to SDS PAGE followed by western blot with 10472-1-AP (PTPN23 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10472-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using PTPN23 Polyclonal antibody (10472-1-AP)

    IP experiment of HeLa using 10472-1-AP (same clone as 10472-1-PBS)

    IP result of anti-PTPN23 (IP:10472-1-AP, 5ug; Detection:10472-1-AP 1:500) with HeLa cells lysate 1000ug. This data was developed using the same antibody clone with 10472-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using PTPN23 Polyclonal antibody (10472-1-AP)

    IHC staining of human colon cancer using 10472-1-AP (same clone as 10472-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10472-1-AP (PTPN23 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10472-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using PTPN23 Polyclonal antibody (10472-1-AP)

    IHC staining of human colon cancer using 10472-1-AP (same clone as 10472-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10472-1-AP (PTPN23 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10472-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using PTPN23 Polyclonal antibody (10472-1-AP)

    IHC staining of human colon cancer using 10472-1-AP (same clone as 10472-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer using 10472-1-AP (PTPN23 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 10472-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using PTPN23 Polyclonal antibody (10472-1-AP)

    IF Staining of HepG2 using 10472-1-AP (same clone as 10472-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using PTPN23 antibody (10472-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 10472-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

PTPN23 Polyclonal antibody, PBS Only

PTPN23 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 10472-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

EC:3.1.3.48, HD PTP, HDPTP, HD-PTP, His domain-containing protein tyrosine phosphatase

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

10472-1-PBS targets PTPN23 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag0765

Product name: Recombinant human PTPN23 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 420-619 aa of BC004881

Sequence: QEKLHLRFCYEAVVRHVEQVLQRHGVPPPCKPLASASISQKNHLPQDSQDLVLGGDVPISSIQATIAKLSIRPPGGLESPVASLPGPAEPPGLPPASLPESTPIPSSSPPPLSSPLPEAPQPKEEPPVPEAPSSGPPSSSLELLASLTPEAFSLDSSLRGKQRMSKHNFLQAHNGQGLRATRPSDDPLSLLDPLWTLNKT

相同性解析による交差性が予測される生物種
Full Name protein tyrosine phosphatase, non-receptor type 23
Calculated molecular weight 179 kDa
Observed molecular weight 179 kDa
GenBank accession numberBC004881
Gene Symbol PTPN23
Gene ID (NCBI) 25930
RRIDAB_2173382
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H3S7
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

PTPN23(Tyrosine-protein phosphatase non-receptor type 23) is also named as HD-PTP,PTP-TD14 and KIAA1471.It encodes a 185-kDa protein which is expressed in human endothelial fromthe umbilical cord and in human Kaposi-spindle cells(PMID:16408268).PTPN23 is a non-transmembrane protein tyrosine phosphatase which contributes to the signal transduction pathways involved in the regulation of cell migration and invasion(PMID:22510412).

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