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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of Jurkat cells using PTPN22 Recombinant antibody (85062-4-RR)

    IF Staining of Jurkat using 85062-4-RR

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using PTPN22 antibody (85062-4-RR, Clone: 242688A2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Flow cytometry (FC) experiment of Jurkat cells using PTPN22 Recombinant antibody (85062-4-RR)

    FC experiment of Jurkat using 85062-4-RR

    1x10^6 Jurkat cells were intracellularly stained with 0.25 ug PTPN22 Recombinant antibody (85062-4-RR, Clone:242688A2) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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PTPN22 Recombinant antibody

PTPN22 Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra), ELISA
Cat No. 85062-4-RR
Clone No.242688A2

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), ELISA

Synonyms

242688A2, EC:3.1.3.48, Hematopoietic cell protein-tyrosine phosphatase 70Z-PEP, LyP, LYP1

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF/ICC detected inJurkat cells
Positive FC (Intra) detected inJurkat cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

85062-4-RR targets PTPN22 in IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen PTPN22 fusion protein Ag2397 相同性解析による交差性が予測される生物種
Full Name protein tyrosine phosphatase, non-receptor type 22 (lymphoid)
Calculated molecular weight 82 kDa, 92 kDa
GenBank accession numberBC017785
Gene Symbol PTPN22
Gene ID (NCBI) 26191
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9Y2R2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PTPN22(Tyrosine-protein phosphatase non-receptor type 22) is also named as LyP,PEP,PTPN8.It belongs to the protein-tyrosine phosphatase family and non-receptor class 4 subfamily.It acts as negative regulator of T-cell receptor (TCR) signaling.Defects in PTPN22 are a cause of susceptibility to systemic lupus erythematosus (SLE). It has some isoforms produced by alternative splicing with calculated molecular mass of 79-92 kDa, and apparent molecular mass of 100-110 kDa.

Protocols

Product Specific Protocols
IF protocol for PTPN22 antibody 85062-4-RRDownload protocol
FC protocol for PTPN22 antibody 85062-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols