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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PTPN20B Polyclonal antibody (19510-1-AP)

    WB analysis using 19510-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 19510-1-AP (PTPN20B antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse tongue tissue using PTPN20 Polyclonal antibody (19510-1-AP)

    IHC staining of mouse tongue using 19510-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse tongue tissue slide using 19510-1-AP (PTPN20 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse spleen tissue using PTPN20 Polyclonal antibody (19510-1-AP)

    IHC staining of mouse spleen using 19510-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 19510-1-AP (PTPN20 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse spleen tissue using PTPN20 Polyclonal antibody (19510-1-AP)

    IHC staining of mouse spleen using 19510-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 19510-1-AP (PTPN20 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse tongue tissue using PTPN20 Polyclonal antibody (19510-1-AP)

    IHC staining of mouse tongue using 19510-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse tongue tissue slide using 19510-1-AP (PTPN20 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse tongue tissue using PTPN20 Polyclonal antibody (19510-1-AP)

    IHC staining of mouse tongue using 19510-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse tongue tissue slide using 19510-1-AP (PTPN20 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using PTPN20 Polyclonal antibody (19510-1-AP)

    IF Staining of A431 using 19510-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using PTPN20 antibody (19510-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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PTPN20 Polyclonal antibody

PTPN20 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 19510-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

PTPN20B, Tyrosine-protein phosphatase non-receptor type 20, PTPN20A, hPTPN20, EC:3.1.3.48

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse tongue tissue, pig tongue tissue, rat tongue tissue
Positive IHC detected inmouse tongue tissue, mouse spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

19510-1-AP targets PTPN20 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name protein tyrosine phosphatase, non-receptor type 20B
Calculated molecular weight 48 kDa
Observed molecular weight52 kDa
GenBank accession numberNM_015605
Gene Symbol PTPN20B
Gene ID (NCBI) 26095
RRIDAB_3669310
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ4JDL3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PTPN20B, also named as PTPN20A, belongs to the protein-tyrosine phosphatase family and Non-receptor class subfamily. PTPN20B is a tyrosine-protein phosphatase targeted to sites of actin polymerization in response of varied extracellular stimuli. PTPN20B has tyrosine phosphatase activity towards various tyrosyl phosphorylated substrates. PTPN20B catalyze the reaction: Protein tyrosine phosphate + H2O = protein tyrosine + phosphate. The antibody can recognize the isoform 1,2,7,8,10,11,13,15.

Protocols

Product Specific Protocols
WB protocol for PTPN20 antibody 19510-1-APDownload protocol
IHC protocol for PTPN20 antibody 19510-1-APDownload protocol
IF protocol for PTPN20 antibody 19510-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols