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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PTPN2 Monoclonal antibody (67388-1-Ig)

    WB analysis using 67388-1-Ig (same clone as 67388-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67388-1-Ig (PTPN2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67388-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using PTPN2 Monoclonal antibody (67388-1-Ig)

    WB analysis using 67388-1-Ig (same clone as 67388-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67388-1-Ig (PTPN2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67388-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of 4T1 cells using PTPN2 Monoclonal antibody (67388-1-Ig)

    WB analysis of 4T1 using 67388-1-Ig (same clone as 67388-1-PBS)

    4T1 cells were subjected to SDS PAGE followed by western blot with 67388-1-Ig (PTPN2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67388-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using PTPN2 Monoclonal antibody (67388-1-Ig)

    IHC staining of human breast cancer using 67388-1-Ig (same clone as 67388-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67388-1-Ig (PTPN2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67388-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using PTPN2 Monoclonal antibody (67388-1-Ig)

    IHC staining of human breast cancer using 67388-1-Ig (same clone as 67388-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67388-1-Ig (PTPN2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67388-1-PBS in a different storage buffer formulation.

PTPN2 Monoclonal antibody, PBS Only

PTPN2 Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 67388-1-PBS
Clone No.2A1D1

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

2A1D1, EC:3.1.3.48, PTN2, PTPN 2, PTPT

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67388-1-PBS targets PTPN2 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag29749

Product name: Recombinant human PTPN2 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-346 aa of BC016727

Sequence: MPTTIEREFEELDTQRRWQPLYLEIRNESHDYPHRVAKFPENRNRNRYRDVSPYDHSRVKLQNAENDYINASLVDIEEAQRSYILTQGPLPNTCCHFWLMVWQQKTKAVVMLNRIVEKESVKCAQYWPTDDQEMLFKETGFSVKLLSEDVKSYYTVHLLQLENINSGETRTISHFHYTTWPDFGVPESPASFLNFLFKVRESGSLNPDHGPAVIHCSAGIGRSGTFSLVDTCLVLMEKGDDINIKQVLLNMRKYRMGLIQTPDQLRFSYMAIIEGAKCIKGDSSIQKRWKELSKEDLSPAFDHSPNKIMTEKYNGNRIGLEEEKLTGDRCTGLSSKMQDTMEENSE

相同性解析による交差性が予測される生物種
Full Name protein tyrosine phosphatase, non-receptor type 2
Calculated molecular weight 49 kDa
Observed molecular weight 45-50 kDa
GenBank accession numberBC016727
Gene Symbol PTPN2
Gene ID (NCBI) 5771
RRIDAB_2882632
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP17706
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

PTPN2(Tyrosine-protein phosphatase non-receptor type 2) is also named as PTPT and belongs to the protein-tyrosine phosphatase family. It may modulate beta-cell apoptosis and/or local release of inflammatory mediators in the course of a viral infection by acting, at least in part, at the pancreatic beta-cell level(PMID: 19825843). This protein has some isoforms produced by alternative splicing with the molecular mass of 40-50 kDa.

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