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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse thymus tissue using PTPN2 Polyclonal antibody (11214-1-AP)

    WB analysis of mouse thymus using 11214-1-AP (same clone as 11214-1-PBS)

    mouse thymus tissue were subjected to SDS PAGE followed by western blot with 11214-1-AP (PTPN2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11214-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HL-60 cells using PTPN2 Polyclonal antibody (11214-1-AP)

    WB analysis of HL-60 using 11214-1-AP (same clone as 11214-1-PBS)

    HL-60 cells were subjected to SDS PAGE followed by western blot with 11214-1-AP (PTPN2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11214-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using PTPN2 Polyclonal antibody (11214-1-AP)

    WB analysis of HeLa using 11214-1-AP (same clone as 11214-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 11214-1-AP (PTPN2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11214-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HL-60 cells using PTPN2 Polyclonal antibody (11214-1-AP)

    WB analysis of HL-60 using 11214-1-AP (same clone as 11214-1-PBS)

    HL-60 cells were subjected to SDS PAGE followed by western blot with 11214-1-AP (PTPN2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11214-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of NIH/3T3 cells using PTPN2 Polyclonal antibody (11214-1-AP)

    WB analysis of NIH/3T3 using 11214-1-AP (same clone as 11214-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 11214-1-AP (PTPN2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11214-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HL-60 cells using PTPN2 Polyclonal antibody (11214-1-AP)

    WB analysis of HL-60 using 11214-1-AP (same clone as 11214-1-PBS)

    HL-60 cells were subjected to SDS PAGE followed by western blot with 11214-1-AP (PTPN2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11214-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HL-60 cells using PTPN2 Polyclonal antibody (11214-1-AP)

    IP experiment of HL-60 using 11214-1-AP (same clone as 11214-1-PBS)

    IP result of anti-PTPN2 (IP:11214-1-AP, 4ug; Detection:11214-1-AP 1:500) with HL-60 cells lysate 4000ug. This data was developed using the same antibody clone with 11214-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using PTPN2 Polyclonal antibody (11214-1-AP)

    IHC staining of human colon cancer using 11214-1-AP (same clone as 11214-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer using 11214-1-AP (PTPN2 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 11214-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using PTPN2 Polyclonal antibody (11214-1-AP)

    IHC staining of human colon cancer using 11214-1-AP (same clone as 11214-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer slide using 11214-1-AP (PTPN2 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 11214-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using PTPN2 Polyclonal antibody (11214-1-AP)

    IHC staining of human colon cancer using 11214-1-AP (same clone as 11214-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer slide using 11214-1-AP (PTPN2 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 11214-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using PTPN2 Polyclonal antibody (11214-1-AP)

    IF Staining of U2OS using 11214-1-AP (same clone as 11214-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using PTPN2 antibody (11214-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 11214-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

PTPN2 Polyclonal antibody, PBS Only

PTPN2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 11214-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

T-cell protein-tyrosine phosphatase, TC PTP, PTPT, PTPN 2, PTN2

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

11214-1-PBS targets PTPN2 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag1709

Product name: Recombinant human PTPN2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-346 aa of BC016727

Sequence: MPTTIEREFEELDTQRRWQPLYLEIRNESHDYPHRVAKFPENRNRNRYRDVSPYDHSRVKLQNAENDYINASLVDIEEAQRSYILTQGPLPNTCCHFWLMVWQQKTKAVVMLNRIVEKESVKCAQYWPTDDQEMLFKETGFSVKLLSEDVKSYYTVHLLQLENINSGETRTISHFHYTTWPDFGVPESPASFLNFLFKVRESGSLNPDHGPAVIHCSAGIGRSGTFSLVDTCLVLMEKGDDINIKQVLLNMRKYRMGLIQTPDQLRFSYMAIIEGAKCIKGDSSIQKRWKELSKEDLSPAFDHSPNKIMTEKYNGNRIGLEEEKLTGDRCTGLSSKMQDTMEENSE

相同性解析による交差性が予測される生物種
Full Name protein tyrosine phosphatase, non-receptor type 2
Calculated molecular weight 49 kDa
Observed molecular weight 45-50 kDa
GenBank accession numberBC016727
Gene Symbol PTPN2
Gene ID (NCBI) 5771
RRIDAB_2173235
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP17706
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

PTPN2(Tyrosine-protein phosphatase non-receptor type 2) is also named as PTPT and belongs to the protein-tyrosine phosphatase family. It may modulate beta-cell apoptosis and/or local release of inflammatory mediators in the course of a viral infection by acting, at least in part, at the pancreatic beta-cell level(PMID: 19825843). This protein has some isoforms produced by alternative splicing with the molecular mass of 40-50 kDa.

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