Validation Data Gallery
Tested Applications
| Positive WB detected in | Jurkat cells, human brain tissue |
| Positive IHC detected in | human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
| IHC | See 2 publications below |
| IF | See 1 publications below |
Product Information
17551-1-AP targets PTPN18 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag11704 Product name: Recombinant human PTPN18 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-351 aa of BC052800 Sequence: MSRSLDSARSFLERLEARGGREGAVLAGEFSKRCERYWAQEQEPLQTGLFCITLIKEKWLNEDIMLRTLKVTFQKESRSVYQLQYMSWPDRGVPSSPDHMLAMVEEARRLQGSGPEPLCVHCSAGCGRTGVLCTVDYVRQLLLTQMIPPDFSLFDVVLKMRKQRPAAVQTEEQYRFLYHTVAQMFCSTLQNASPHYQNIKENCAPLYDDALFLRTPQALLAIPRPPGGVLRSISVPGSPGHAMADTYAVVQKRGAPAGAGSGTQTGTGTGARSAEEAPLYSKVTPRAQRPGAHAEDARGTLPGRVPADQSPAGSGAYEDVAGGAQTGGLGFNLRIGRPKGPRDPPAEWTRV 相同性解析による交差性が予測される生物種 |
| Full Name | protein tyrosine phosphatase, non-receptor type 18 (brain-derived) |
| Calculated molecular weight | 460 aa, 50 kDa |
| Observed molecular weight | 50 kDa |
| GenBank accession number | BC052800 |
| Gene Symbol | PTPN18 |
| Gene ID (NCBI) | 26469 |
| RRID | AB_2284599 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q99952 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
PTPN18(Tyrosine-protein phosphatase non-receptor type 18) is also named as BDP1 and belongs to the protein-tyrosine phosphatase family. The PTPN18 cDNA encodes a deduced 459-amino acid protein with no transmembrane domain and a calculated molecular weight of 50 kD. PTPN18 has a PEST motif and shares the greatest homology with members of the PTP-PEST gene family. Northern blot analysis indicated that PTPN18 is expressed not only in brain, but also in colon and several different tumor-derived cell lines.(PMID:8950995). It has 2 isoforms produced by alternative splicing.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for PTPN18 antibody 17551-1-AP | Download protocol |
| WB protocol for PTPN18 antibody 17551-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Physiol Biochem Prognostic Value of Phosphotyrosine Phosphatases in Hepatocellular Carcinoma. | ||
Mol Cancer Res Hepatitis B virus X protein represses expression of tumor suppressor PTPN18 in hepatocellular carcinoma | ||
Clin Exp Pharmacol Physiol Downregulation of PTPN18 can inhibit proliferation and metastasis and promote apoptosis of endometrial cancer. |
