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Validation Data Gallery

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  • Western Blot (WB) analysis of Sp2/0 cells using PTP1B Polyclonal antibody (11334-1-AP)

    WB analysis of Sp2/0 using 11334-1-AP

    Sp2/0 cells were subjected to SDS PAGE followed by western blot with 11334-1-AP (PTP1B antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using PTP1B Polyclonal antibody (11334-1-AP)

    WB analysis of Jurkat using 11334-1-AP

    WB result of PTP1B antibody (11334-1-AP; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PTP1B transfected Jurkat cells.

  • Western Blot (WB) analysis of various lysates using PTP1B Polyclonal antibody (11334-1-AP)

    WB analysis using 11334-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11334-1-AP (PTP1B antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using PTP1B Polyclonal antibody (11334-1-AP)

    WB analysis using 11334-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11334-1-AP (PTP1B antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using PTP1B Polyclonal antibody (11334-1-AP)

    WB analysis using 11334-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11334-1-AP (PTP1B antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of THP-1 cells using PTP1B Polyclonal antibody (11334-1-AP)

    WB analysis of THP-1 using 11334-1-AP

    THP-1 cells were subjected to SDS PAGE followed by western blot with 11334-1-AP (PTP1B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of A431 cells using PTP1B Polyclonal antibody (11334-1-AP)

    IP experiment of A431 using 11334-1-AP

    IP result of anti-PTP1B (IP:11334-1-AP, 4ug; Detection:11334-1-AP 1:500) with A431 cells lysate 2400ug.

  • Immunohistochemistry (IHC) staining of mouse placenta tissue using PTPN1/PTP1B Polyclonal antibody (11334-1-AP)

    IHC staining of mouse placenta using 11334-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse placenta tissue slide using 11334-1-AP (PTPN1/PTP1B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse placenta tissue using PTPN1/PTP1B Polyclonal antibody (11334-1-AP)

    IHC staining of mouse placenta using 11334-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse placenta tissue slide using 11334-1-AP (PTPN1/PTP1B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of BxPC-3 cells using PTP1B Polyclonal antibody (11334-1-AP)

    IF Staining of BxPC-3 using 11334-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed BxPC-3 cells using PTP1B antibody (11334-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using PTPN1/PTP1B Polyclonal antibody (11334-1-AP)

    IF Staining of U2OS using 11334-1-AP

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using PTPN1/PTP1B antibody (11334-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

  • Featured Product
  • KD/KO Validated

PTPN1/PTP1B Polyclonal antibody

PTPN1/PTP1B Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 11334-1-AP
Publications(60)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Synonyms

PTP1B, PTPN1, EC:3.1.3.48, Protein-tyrosine phosphatase 1B, PTP 1B

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inSp2/0 cells, HeLa cells, mouse liver tissue, THP-1 cells, Jurkat cells, C6 cells, NIH/3T3 cells, rat liver tissue, mouse placenta tissue
Positive IP detected inA431 cells
Positive IHC detected inmouse placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inBxPC-3 cells, U2OS cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11334-1-AP targets PTPN1/PTP1B in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, monkey
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag1878

Product name: Recombinant human PTPN1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-435 aa of BC015660

Sequence: MEMEKEFEQIDKSGSWAAIYQDIRHEASDFPCRVAKLPKNKNRNRYRDVSPFDHSRIKLHQEDNDYINASLIKMEEAQRSYILTQGPLPNTCGHFWEMVWEQKSRGVVMLNRVMEKGSLKCAQYWPQKEEKEMIFEDTNLKLTLISEDIKSYYTVRQLELENLTTQETREILHFHYTTWPDFGVPESPASFLNFLFKVRESGSLSPEHGPVVVHCSAGIGRSGTFCLADTCLLLMDKRKDPSSVDIKKVLLEMRKFRMGLIQTADQLRFSYLAVIEGAKFIMGDSSVQDQWKELSHEDLEPPPEHIPPPPRPPKRILEPHNGKCREFFPNHQWVKEETQEDKDCPIKEEKGSPLNAAPYGIESMSQDTEVRSRVVGGSLRGAQAASPAKGEPSLPEKDEDHALSYWKPFLVNMCVATVLTAGAYLCYRFLFNSNT

相同性解析による交差性が予測される生物種
Full Name protein tyrosine phosphatase, non-receptor type 1
Calculated molecular weight 435 aa, 50 kDa
Observed molecular weight 45-55 kDa
GenBank accession numberBC015660
Gene Symbol PTP1B
Gene ID (NCBI) 5770
RRIDAB_10642566
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP18031
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PTPN1 (protein tyrosine phosphatase, non-receptor type 1) is also named as PTP1B or PTP-1B, and belongs to the protein-tyrosine phosphatase family and non-receptor class 1 subfamily. PTPN1 is involved in the regulation of several important physiological pathways, which regulates both INS and leptin signaling, and interacts with the epidermal-and platelet-derived growth factor receptors (PMID: 20101100). The gene might harbor variants influencing susceptibility to INS resistance and type 2 diabetes (PMID:15919813). The molecular mass of PTPN1 is 50 kDa, but sometimes some truncated forms can be detected as 48, 46, 39, 37 kDa due to non-specific proteolytic cleavage of PTP1B in different cells (PMID: 18253097).

Protocols

Product Specific Protocols
WB protocol for PTPN1/PTP1B antibody 11334-1-APDownload protocol
IF protocol for PTPN1/PTP1B antibody 11334-1-APDownload protocol
IHC protocol for PTPN1/PTP1B antibody 11334-1-APDownload protocol
IP protocol for PTPN1/PTP1B antibody 11334-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,IF

Nat Commun

BACH1 controls hepatic insulin signaling and glucose homeostasis in mice

Authors - Jiayu Jin
View Article
mouse,humanWB,IP

Sci Transl Med

The m6A demethylase FTO links TLR7 to mitochondrial oxidation driving age-associated B cell formation in systemic lupus erythematosus

Authors - Qin Zeng
View Article
humanWB

J Exp Clin Cancer Res

Ficolin 3 promotes ferroptosis in HCC by downregulating IR/SREBP axis-mediated MUFA synthesis

Authors - Yanmei Yuan
View Article
humanWB,IF

J Exp Clin Cancer Res

HERC2 promotes inflammation-driven cancer stemness and immune evasion in hepatocellular carcinoma by activating STAT3 pathway

Authors - Yunzhi Liu
View Article
rat,humanWB,IHC

Cell Death Dis

The CREB/KMT5A complex regulates PTP1B to modulate high glucose-induced endothelial inflammatory factor levels in diabetic nephropathy.

Authors - Ting Huang
  • KD Validated
View Article
mouseWB

Phytomedicine

Zedoarondiol regulates steatosis and inflammation in alcoholic liver injury via PTP1B-target mechanisms.

Authors - Jinru Zhu
View Article
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