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- KD/KO Validated
PTPIP51 Polyclonal antibody
PTPIP51 Polyclonal Antibody for IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (3)
Applications
WB, IP, IHC, IF, COIP, ELISA
Conjugate
Unconjugated
Cat no : 20641-1-AP
Synonyms
Validation Data Gallery
Applications:
Tested Applications
| Positive WB detected in | HEK-293 cells, Jurkat cells |
| Positive IP detected in | HEK-293 cells |
| Positive IHC detected in | human skin tissue, human cervical cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF) | IF : 1:10-1:100 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 6 publications below |
| IHC | See 2 publications below |
| IF | See 4 publications below |
| CoIP | See 1 publications below |
Product Information
The immunogen of 20641-1-AP is PTPIP51 Fusion Protein expressed in E. coli.
| Tested Reactivity | human |
| Cited Reactivity | human, rat, mouse, monkey |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | PTPIP51 fusion protein Ag14708 相同性解析による交差性が予測される生物種 |
| Full Name | family with sequence similarity 82, member A2 |
| Calculated molecular weight | 470 aa, 52 kDa |
| Observed molecular weight | 52-60 kDa |
| GenBank accession number | BC008970 |
| Gene symbol | FAM82A2 |
| Gene ID (NCBI) | 55177 |
| RRID | AB_10695187 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for PTPIP51 antibody 20641-1-AP | Download protocol |
| IHC protocol for PTPIP51 antibody 20641-1-AP | Download protocol |
| IF protocol for PTPIP51 antibody 20641-1-AP | Download protocol |
| IP protocol for PTPIP51 antibody 20641-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Cell Biol MIROs and DRP1 drive mitochondrial-derived vesicle biogenesis and promote quality control.
| ||
Mol Cell The ER-Localized Transmembrane Protein EPG-3/VMP1 Regulates SERCA Activity to Control ER-Isolation Membrane Contacts for Autophagosome Formation. | ||
J Cell Biol Atlastin 2/3 regulate ER targeting of the ULK1 complex to initiate autophagy. | ||
J Cell Sci The role of Mfn2 in the structure and function of endoplasmic reticulum-mitochondrial tethering in vivo. | ||
Sci Rep Proteomics-Based Approach Identifies Altered ER Domain Properties by ALS-Linked VAPB Mutation. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Tom (Verified Customer) (03-23-2021) | Cos7 cells fixed in 4% PFA, perm in 0.1% Triton then blocked in 1% BSA. Mitochondria also labelled with TOMM20. Cells incubated with primary antibody (1/100) for 1 hour.
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