Validation Data Gallery
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Tested Applications
| Positive WB detected in | mouse brain tissue, Jurkat cells |
| Positive IHC detected in | human medulloblastoma tissue, human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
Product Information
10044-2-AP targets PYK2 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag0002 Product name: Recombinant human PTK2B protein Source: e coli.-derived, Unknow Tag: N/A Domain: Gst-PYK1-C of BC036651 Sequence: 相同性解析による交差性が予測される生物種 |
| Full Name | PTK2B protein tyrosine kinase 2 beta |
| Calculated molecular weight | 120 kDa |
| Observed molecular weight | 116 kDa |
| GenBank accession number | BC036651 |
| Gene Symbol | PTK2B |
| Gene ID (NCBI) | 2185 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q14289 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Proline-rich tyrosine kinase 2 (Pyk2; also known as CAK, RAFTK and CADTK) is a cytoplasmic tyrosine kinase implicated to play a role in several introcellular signaling pathways. It is expressed in many cells and tissues and migrated as a 130 kDa band in Western Blotting analysis.