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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PTGIS Recombinant monoclonal antibody (85810-1-RR)

    WB analysis using 85810-1-RR (same clone as 85810-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85810-1-RR (PTGIS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85810-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using PTGIS Recombinant monoclonal antibody (85810-1-RR)

    IF Staining of U2OS using 85810-1-RR (same clone as 85810-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using PTGIS antibody (85810-1-RR, Clone: 243128C2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 85810-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 85810-1-RR

    Affinity and Kinetic Characterization of 85810-1-RR

    Biolayer interferometry (BLl) kinetic assays of 85810-1-RR against Human PTGIS were performed. The affinity constant is below 1 pM.

PTGIS Recombinant monoclonal antibody, PBS Only

PTGIS Uni-rAb® Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 85810-1-PBS
Clone No.243128C2

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

CYP8, CYP8A1, EC:4.2.1.152, EC:5.3.99.4, Hydroperoxy icosatetraenoate dehydratase

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

85810-1-PBS targets PTGIS in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag25817

Product name: Recombinant human PTGIS protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 100-285 aa of BC101809

Sequence: AIFLMERIFDVQLPHYSPSDEKARMKLTLLHRELQALTEAMYTNLHAVLLGDATEAGSGWHEMGLLDFSYSFLLRAGYLTLYGIEALPRTHESQAQDRVHSADVFHTFRQLDRLLPKLARGSLSVGDKDHMCSVKSRLWKLLSPARLARRAHRSKWLESYLLHLEEMGVSEEMQARALVLQLWATQ

相同性解析による交差性が予測される生物種
Full Name prostaglandin I2 (prostacyclin) synthase
Calculated molecular weight 500 aa, 57 kDa
Observed molecular weight50-57 kDa
GenBank accession numberBC101809
Gene Symbol PTGIS
Gene ID (NCBI) 5740
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ16647
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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