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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PTGIS Polyclonal antibody

PTGIS Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 27061-1-AP

Synonyms

CYP8, CYP8A1, PGIS, Prostacyclin synthase, Prostaglandin I2 synthase, PTGI, PTGIS

Validation Data Gallery

Applications:
  • WB analysis using 27061-1-AP

    WB analysis using 27061-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27061-1-AP (PTGIS antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse liver using 27061-1-AP

    IHC staining of mouse liver using 27061-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 27061-1-AP (PTGIS antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with .

  • IHC staining of mouse liver using 27061-1-AP

    IHC staining of mouse liver using 27061-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 27061-1-AP (PTGIS antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with .

  • IF Staining of Saos-2 using 27061-1-AP

    IF Staining of Saos-2 using 27061-1-AP

    Immunofluorescent analysis of (4% PFA) fixed Saos-2 cells using PTGIS antibody (27061-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

Tested Applications

Positive WB detected inmouse lung tissue, mouse testis tissue, rat lung tissue
Positive IHC detected inmouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inSaos-2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

27061-1-AP targets PTGIS in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PTGIS fusion protein Ag25817 相同性解析による交差性が予測される生物種
Full Name prostaglandin I2 (prostacyclin) synthase
Calculated molecular weight 500 aa, 57 kDa
Observed molecular weight50-57 kDa
GenBank accession numberBC101809
Gene symbol PTGIS
Gene ID (NCBI) 5740
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for PTGIS antibody 27061-1-APDownload protocol
IHC protocol for PTGIS antibody 27061-1-APDownload protocol
IF protocol for PTGIS antibody 27061-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols