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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PTGIR Polyclonal antibody (30657-1-AP)

    WB analysis using 30657-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30657-1-AP (PTGIR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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PTGIR Polyclonal antibody

PTGIR Polyclonal Antibody for WB, ELISA
Cat No. 30657-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

Prostanoid IP receptor, Prostaglandin I2 receptor, Prostacyclin receptor, PRIPR, PGI2 receptor

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHepG2 cells, SGC-7901 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

30657-1-AP targets PTGIR in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PTGIR fusion protein Ag30653 相同性解析による交差性が予測される生物種
Full Name prostaglandin I2 (prostacyclin) receptor (IP)
Calculated molecular weight 386 aa, 41 kDa
Observed molecular weight 41-45kDa
GenBank accession numberBC075814
Gene Symbol PTGIR
Gene ID (NCBI) 5739
RRIDAB_3669746
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP43119
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Prostaglandin I2 receptor (PTGIR), also known as IP, is a seven-transmembrane receptor that binds G proteins leading to activation of adenylyl cyclase and an increase in cAMP formation. PTGIR is involved in insulin secretion in pancreatic beta-cells and in permselectivity in glomerular podocytes.

Protocols

Product Specific Protocols
WB protocol for PTGIR antibody 30657-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols