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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PTGES2 Recombinant antibody (85419-4-RR)

    WB analysis using 85419-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85419-4-RR (PTGES2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using PTGES2 Recombinant antibody (85419-4-RR)

    WB analysis using 85419-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85419-4-RR (PTGES2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using PTGES2 Recombinant monoclonal antibody (85419-4-RR)

    IHC staining of human colon cancer using 85419-4-RR

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 85419-4-RR (PTGES2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using PTGES2 Recombinant monoclonal antibody (85419-4-RR)

    IHC staining of human colon cancer using 85419-4-RR

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 85419-4-RR (PTGES2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using PTGES2 Recombinant monoclonal antibody (85419-4-RR)

    IHC staining of human colon cancer using 85419-4-RR

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 85419-4-RR (PTGES2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using PTGES2 Recombinant antibody (85419-4-RR)

    IF Staining of HepG2 using 85419-4-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PTGES2 antibody (85419-4-RR, Clone: 242930B4 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PTGES2 Recombinant antibody (85419-4-RR)

    IF Staining of HeLa using 85419-4-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PTGES2 antibody (85419-4-RR, Clone: 242930B4 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 85419-4-RR

    Affinity and Kinetic Characterization of 85419-4-RR

    Biolayer interferometry (BLl) kinetic assays of 85419-4-RR against Human PTGES2 were performed. The affinity constant is 0.973 nM.

PTGES2 Recombinant monoclonal antibody

PTGES2 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 85419-4-RR
Clone No.242930B4

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

242930B4, C9orf15, EC:5.3.99.3, GBF1, Membrane-associated prostaglandin E synthase-2

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, mouse heart tissue, HepG2 cells, HEK-293 cells, Jurkat cells, rat heart tissue, pig heart tissue
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

85419-4-RR targets PTGES2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag1329

Product name: Recombinant human PTGES2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-377 aa of BC011613

Sequence: MDPAARVVRALWPGGCALAWRLGGRPQPLLPTQSRAGFAGAAGGPSPVAAARKGSPRLLGAAALALGGALGLYHTARWHLRAQDLHAERSAAQLSLSSRLQLTLYQYKTCPFCSKVRAFLDFHALPYQVVEVNPVRRAEIKFSSYRKVPILVAQEGESSQQLNDSSVIISALKTYLVSGQPLEEIITYYPAMKAVNEQGKEVTEFGNKYWLMLNEKEAQQVYGGKEARTEEMKWRQWADDWLVHLISPNVYRTPTEALASFDYIVREGKFGAVEGAVAKYMGAAAMYLISKRLKSRHRLQDNVREDLYEAADKWVAAVGKDRPFMGGQKPNLADLAVYGVLRVMEGLDAFDDLMQHTHIQPWYLRVERAITEASPAH

相同性解析による交差性が予測される生物種
Full Name prostaglandin E synthase 2
Calculated molecular weight 42 kDa
Observed molecular weight30-35 kDa
GenBank accession numberBC011613
Gene Symbol PTGES2
Gene ID (NCBI) 80142
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ9H7Z7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PTGES2 (Prostaglandin E synthase 2) catalyzes the conversion of unstable intermediate of prostaglandin E2 H2 (PGH2) into the more stable prostaglandin E2 (PGE2) form. Its predicted size is 42 kDa, but it was detected one band of 35 kDa in the prostate and two bands of 30 kDa, 50 kDa in liver by western blot (PMID:17566096). PTGES2 protein is a homotetramer, whereas two PTGES2 molecules each form a strongly interacting dimer(PMID:19268562).

Protocols

Product Specific Protocols
IF protocol for PTGES2 antibody 85419-4-RRDownload protocol
IHC protocol for PTGES2 antibody 85419-4-RRDownload protocol
WB protocol for PTGES2 antibody 85419-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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