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Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human brain tissue using PTGER3 Polyclonal antibody (14357-1-AP)

    IHC staining of human brain using 14357-1-AP (same clone as 14357-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 14357-1-AP (PTGER3 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 14357-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human brain tissue using PTGER3 Polyclonal antibody (14357-1-AP)

    IHC staining of human brain using 14357-1-AP (same clone as 14357-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 14357-1-AP (PTGER3 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 14357-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

14357-1-PBS targets PTGER3 in IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PTGER3 fusion protein Ag5711 相同性解析による交差性が予測される生物種
Full Name prostaglandin E receptor 3 (subtype EP3)
Calculated molecular weight 43 kDa
GenBank accession numberBC024229
Gene Symbol PTGER3
Gene ID (NCBI) 5733
RRIDAB_2237964
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP43115
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

PTGER3 (Prostaglandin E Receptor 3), also known as EP3, is a G protein-coupled receptor (GPCR). Activates pathways involved in proliferation, migration, and apoptosis, often via cross-talk with PI3K-AKT and MAPK cascades (PMID: 38566528). High PTGER3 levels drive tumorigenesis, angiogenesis, and cisplatin resistance via the Ras-MAPK/Erk-ETS1-ELK1/CFTR1 axis (PMID: 30655206).