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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using PTGDS Polyclonal antibody (10754-2-AP)

    WB analysis of HeLa using 10754-2-AP (same clone as 10754-2-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 10754-2-AP (PTGDS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10754-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using PTGDS Polyclonal antibody (10754-2-AP)

    WB analysis using 10754-2-AP (same clone as 10754-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 10754-2-AP (PTGDS antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10754-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human kidney tissue using PTGDS Polyclonal antibody (10754-2-AP)

    WB analysis of human kidney using 10754-2-AP (same clone as 10754-2-PBS)

    human kidney tissue were subjected to SDS PAGE followed by western blot with 10754-2-AP (PTGDS antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10754-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human colon tissue using PTGDS Polyclonal antibody (10754-2-AP)

    WB analysis of human colon using 10754-2-AP (same clone as 10754-2-PBS)

    human colon tissue were subjected to SDS PAGE followed by western blot with 10754-2-AP (PTGDS antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10754-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of PC-3 cells using PTGDS Polyclonal antibody (10754-2-AP)

    WB analysis of PC-3 using 10754-2-AP (same clone as 10754-2-PBS)

    PC-3 cells were subjected to SDS PAGE followed by western blot with 10754-2-AP (PTGDS antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10754-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human heart tissue using PTGDS Polyclonal antibody (10754-2-AP)

    WB analysis of human heart using 10754-2-AP (same clone as 10754-2-PBS)

    human heart tissue were subjected to SDS PAGE followed by western blot with 10754-2-AP (PTGDS antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10754-2-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using PTGDS Polyclonal antibody (10754-2-AP)

    IP experiment of mouse brain using 10754-2-AP (same clone as 10754-2-PBS)

    IP result of anti-PTGDS (IP:10754-2-AP, 4ug; Detection:10754-2-AP 1:600) with mouse brain tissue lysate 1840 ug. This data was developed using the same antibody clone with 10754-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using PTGDS Polyclonal antibody (10754-2-AP)

    IHC staining of human lung cancer using 10754-2-AP (same clone as 10754-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer using 10754-2-AP (PTGDS antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 10754-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using PTGDS Polyclonal antibody (10754-2-AP)

    IHC staining of human lung cancer using 10754-2-AP (same clone as 10754-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer using 10754-2-AP (PTGDS antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 10754-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using PTGDS Polyclonal antibody (10754-2-AP)

    IHC staining of mouse brain using 10754-2-AP (same clone as 10754-2-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10754-2-AP (PTGDS antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10754-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using PTGDS Polyclonal antibody (10754-2-AP)

    IHC staining of mouse brain using 10754-2-AP (same clone as 10754-2-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10754-2-AP (PTGDS antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10754-2-PBS in a different storage buffer formulation.

PTGDS Polyclonal antibody, PBS Only

PTGDS Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 10754-2-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

LPGDS, Lipocalin-type prostaglandin-D synthase, Glutathione-independent PGD synthase, EC:5.3.99.2, Cerebrin-28

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

10754-2-PBS targets PTGDS in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag1163

Product name: Recombinant human PGDS protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-190 aa of BC005939

Sequence: MATHHTLWMGLALLGVLGDLQAAPEAQVSVQPNFQQDKFLGRWFSAGLASNSSWLREKKAALSMCKSVVAPATDGGLNLTSTFLRKNQCETRTMLLQPAGSLGSYSYRSPHWGSTYSVSVVETDYDQYALLYSQGSKGPGEDFRMATLYSRTQTPRAELKEKFTAFCKAQGFTEDTIVFLPQTDKCMTEQ

相同性解析による交差性が予測される生物種
Full Name prostaglandin D2 synthase 21kDa (brain)
Calculated molecular weight 21 kDa
Observed molecular weight 28 kDa
GenBank accession numberBC005939
Gene Symbol PTGDS
Gene ID (NCBI) 5730
RRIDAB_2174610
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP41222
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Glycoprotein prostaglandin D2 synthase (PTGDS), also known as L-PGDS, PGDS, and LPGDS, is a member of the lipocalin superfamily and plays dual roles in prostaglandins metabolism and lipid transport (PMID: 34743203). PTGD2 functions as a neuromodulator as well as a trophic factor in the central nervous system. PTGD2 is also involved in smooth muscle contraction/relaxation and is a potent inhibitor of platelet aggregation. PTGDS is abundantly expressed in the brain and heart and has also been found in testis, prostate, kidney, spleen and leukocytes. It can be found as a collection of monomeric glycoforms with molecular weights in the 27-34 kDa range (PMID: 32430846).

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