Validation Data Gallery
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Tested Applications
| Positive WB detected in | mouse embryo tissue, HEK-293 cells, rat heart tissue, rat kidney tissue |
| Positive IP detected in | HEK-293 cells |
| Positive IF/ICC detected in | HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 4 publications below |
| CoIP | See 1 publications below |
| RIP | See 1 publications below |
Product Information
25158-1-AP targets PTCD3 in WB, IF/ICC, IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag18330 Product name: Recombinant human PTCD3 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 368-689 aa of BC011832 Sequence: SLATYHHIIRLFDQPGDPLKRSSFIIYDIMNELMGKRFSPKDPDDDKFFQSAMSICSSLRDLELAYQVHGLLKTGDNWKFIGPDQHRNFYYSKFFDLICLMEQIDVTLKWYEDLIPSAYFPHSQTMIHLLQALDVANRLEVIPKIWKDSKEYGHTFRSDLREEILMLMARDKHPPELQVAFADCAADIKSAYESQPIRQTAQDWPATSLNCIAILFLRAGRTQEAWKMLGLFRKHNKIPRSELLNELMDSAKVSNSPSQAIEVVELASAFSLPICEGLTQRVMSDFAINQEQKEALSNLTALTSDSDTDSSSDSDSDTSEGK 相同性解析による交差性が予測される生物種 |
| Full Name | Pentatricopeptide repeat domain 3 |
| Calculated molecular weight | 689 aa, 79 kDa |
| Observed molecular weight | 78-80 kDa |
| GenBank accession number | BC011832 |
| Gene Symbol | PTCD3 |
| Gene ID (NCBI) | 55037 |
| RRID | AB_2879931 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96EY7 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for PTCD3 antibody 25158-1-AP | Download protocol |
| IP protocol for PTCD3 antibody 25158-1-AP | Download protocol |
| WB protocol for PTCD3 antibody 25158-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
iScience Supernumerary proteins of the human mitochondrial ribosomal small subunit are integral for assembly and translation | ||
Cell Death Dis MRPS31 loss is a key driver of mitochondrial deregulation and hepatocellular carcinoma aggressiveness. | ||
Biomedicines Mitochondrial Ribosome Dysfunction in Human Alveolar Type II Cells in Emphysema | ||
J Gen Virol Inhibition of human cytomegalovirus replication by interferon alpha can involve multiple anti-viral factors | ||
FASEB J Epigenetic Activation of PTCD3 Promotes CRC Glutamine Metabolism and Metastasis via IGF2BP2-Mediated SLC38A2 m6A Modification |
