Validation Data Gallery
Tested Applications
| Positive WB detected in | Daudi cells, HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
26473-1-AP targets PTCD2 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag24115 Product name: Recombinant human PTCD2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 153-388 aa of NM_001284403 Sequence: MKDQHLRGFFSDSTSFNILMDMLFIKGKYKSALQVLIEMKNQDVKFTKDTYVLAFAICYKLNSPESFKICTTLREEALLKGEILSRRASCFAVALALNQNEMAKAVSIFSQIMNPESIACINLNIIIHIQSNMLENLIKTLKNAAEGNLSKFVKRHVFSEEVLAKVREKVKDVPALVAKFDEIYGTLHITGQVTTDSLDAVLCHTPRDRKSHTLLLNKRMVSRRTFQPLSQSLLAE 相同性解析による交差性が予測される生物種 |
| Full Name | pentatricopeptide repeat domain 2 |
| Observed molecular weight | 33 kDa, 50 kDa |
| GenBank accession number | NM_001284403 |
| Gene Symbol | PTCD2 |
| Gene ID (NCBI) | 79810 |
| RRID | AB_3669538 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8WV60 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
PTCD2 (Pentatricopeptide repeat-containing protein 2), a mysterious protein whose function was unknown despite the available data. Its mitochondrial localization and PPR family membership suggested its possible biological role in regulation of mitochondrial gene expression. (PMID: 36430722) This antibody recognizes three isomers of PTCD2, including isomer 1 (≈ 44 kDa), isomer 2 (≈ 24 kDa) and isomer 3 (≈ 31 kDa). Since the protein has two post-translational modifications, phosphorylation and ubiquitination, the actual measured molecular weight will be larger than the predicted value.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for PTCD2 antibody 26473-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |