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  • Featured Product
  • KD/KO Validated

PTBP1 Polyclonal antibody

PTBP1 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, RIP, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 12582-1-AP

Synonyms

HNRNP I, HNRNPI, HNRPI, pPTB, PTB, PTB 1, PTB T, PTB2, PTB3, PTB4, PTBP1

Validation Data Gallery

Applications:
  • WB analysis using 12582-1-AP

    WB analysis using 12582-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12582-1-AP (PTBP1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 12582-1-AP

    WB analysis of HeLa using 12582-1-AP

    WB result of PTBP1 antibody (12582-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PTBP1 transfected HeLa cells.

  • WB analysis of rat heart using 12582-1-AP

    WB analysis of rat heart using 12582-1-AP

    rat heart tissue were subjected to SDS PAGE followed by western blot with 12582-1-AP (PTBP1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse lung using 12582-1-AP

    WB analysis of mouse lung using 12582-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 12582-1-AP (PTBP1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human prostate cancer using 12582-1-AP

    IHC staining of human prostate cancer using 12582-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12582-1-AP (PTBP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PTBP1 antibody included
  • IHC staining of mouse brain using 12582-1-AP

    IHC staining of mouse brain using 12582-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12582-1-AP (PTBP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PTBP1 antibody included
  • IHC staining of human prostate cancer using 12582-1-AP

    IHC staining of human prostate cancer using 12582-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 12582-1-AP (PTBP1 antibody) at dilution of 1:50 (under 10x lens).

    Ready-to-use IHC kits available with PTBP1 antibody included
  • IHC staining of human prostate cancer using 12582-1-AP

    IHC staining of human prostate cancer using 12582-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12582-1-AP (PTBP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PTBP1 antibody included
  • IHC staining of mouse brain using 12582-1-AP

    IHC staining of mouse brain using 12582-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12582-1-AP (PTBP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PTBP1 antibody included
  • IHC staining of human pancreas using 12582-1-AP

    IHC staining of human pancreas using 12582-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas slide using 12582-1-AP (PTBP1 Antibody) at dilution of 1:50.

    Ready-to-use IHC kits available with PTBP1 antibody included
  • IHC staining of human pancreas using 12582-1-AP

    IHC staining of human pancreas using 12582-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas slide using 12582-1-AP (PTBP1 Antibody) at dilution of 1:50.

    Ready-to-use IHC kits available with PTBP1 antibody included
  • IF Staining of mouse brain using 12582-1-AP

    IF Staining of mouse brain using 12582-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 12582-1-AP (PTBP1 antibody), at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of mouse brain using 12582-1-AP

    IF Staining of mouse brain using 12582-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 12582-1-AP (PTBP1 antibody), at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inA431 cells, HeLa cells, rat heart tissue, mouse lung tissue, BxPC-3 cells, Jurkat cells, K-562 cells
Positive IHC detected inhuman prostate cancer tissue, human pancreas tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse brain tissue
Planning an IHC experiment? We recommend our IHCeasy PTBP1 Ready-To-Use IHC Kit. PTBP1 primary antibody included.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

12582-1-AP targets PTBP1 in WB, RIP, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, rat, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PTBP1 fusion protein Ag3277 相同性解析による交差性が予測される生物種
Full Name polypyrimidine tract binding protein 1
Calculated molecular weight 60 kDa
Observed molecular weight 60 kDa
GenBank accession numberBC013694
Gene symbol PTBP1
Gene ID (NCBI) 5725
RRIDAB_2176402
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Polypyrimidine tract-binding protein (PTBP1), also known as heterogeneous nuclear ribonucleoprotein type I (hnRNP I), is a nuclear protein that binds pre-mRNAs in specific regions of the hnRNA-protein complexes sensitive to micrococcal nuclease. It involves in pre-mRNA splicing and the regulation of alternative splicing events. PTBP1 promotes RNA looping when bound to two separate polypyrimidine tracts in the same pre-mRNA, and promotes the binding of U2 snRNP to pre-mRNA. Besides, it can cooperate with RAVER1 to modulate switching between mutually exclusive exons during maturation of the TPM1 pre-mRNA, and represses the splicing of MAPT/Tau exon 10

Protocols

Product Specific Protocols
WB protocol for PTBP1 antibody 12582-1-APDownload protocol
IHC protocol for PTBP1 antibody 12582-1-APDownload protocol
IF protocol for PTBP1 antibody 12582-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Cancer

SULT1A1-dependent sulfonation of alkylators is a lineage-dependent vulnerability of liver cancers

Authors - Lei Shi
View Article
humanWB

Nat Cell Biol

Arg-tRNA synthetase links inflammatory metabolism to RNA splicing and nuclear trafficking via SRRM2

Authors - Haissi Cui
View Article
humanRIP

Blood

circMYBL2, a circRNA from MYBL2, regulates FLT3 translation by recruiting PTBP1 to promote FLT3-ITD AML progression.

Authors - Yu-Meng Sun
View Article
humanWB

Nat Commun

Systematic characterization of cancer transcriptome at transcript resolution

Authors - Wei Hu
View Article
ratWB

Nucleic Acids Res

PMID: 30295850

Authors - Zonghong Li
View Article
humanWB

Dev Cell

Genome-wide analysis of pseudogenes reveals HBBP1's human-specific essentiality in erythropoiesis and implication in β-thalassemia.

Authors - Yanni Ma
View Article