Validation Data Gallery
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Tested Applications
| Positive IHC detected in | human lung tissue, human heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
Product Information
20921-1-AP targets PTAFR in WB, IHC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag15128 Product name: Recombinant human PTAFR protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 245-342 aa of BC013816 Sequence: FVPHHVVQLPWTLAELGFQDSKFHQAINDAHQVTLCLLSTNCVLDPVIYCFLTKKFRKHLTEKFYSMRSSRKCSRATTDTVTEVVVPFNQIPGNSLKN 相同性解析による交差性が予測される生物種 |
| Full Name | platelet-activating factor receptor |
| Calculated molecular weight | 342 aa, 39 kDa |
| GenBank accession number | BC013816 |
| Gene Symbol | PTAFR |
| Gene ID (NCBI) | 5724 |
| RRID | AB_2878766 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P25105 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for PTAFR antibody 20921-1-AP | Download protocol |
| IHC protocol for PTAFR antibody 20921-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Struct Mol Biol Structural basis for signal recognition and transduction by platelet-activating-factor receptor. | ||
Gut Pathog Virulence factors and mechanisms of paediatric pneumonia caused by Enterococcus faecalis |
