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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PSMG3 Monoclonal antibody (67466-1-Ig)

    WB analysis using 67466-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67466-1-Ig (PSMG3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using PSMG3 Monoclonal antibody (67466-1-Ig)

    WB analysis using 67466-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67466-1-Ig (PSMG3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • Western Blot (WB) analysis of K-562 cells using PSMG3 Monoclonal antibody (67466-1-Ig)

    WB analysis of K-562 using 67466-1-Ig

    K-562 cells were subjected to SDS PAGE followed by western blot with 67466-1-Ig (PSMG3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using PSMG3 Monoclonal antibody (67466-1-Ig)

    WB analysis using 67466-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67466-1-Ig (PSMG3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HL-60 cells using PSMG3 Monoclonal antibody (67466-1-Ig)

    WB analysis of HL-60 using 67466-1-Ig

    HL-60 cells were subjected to SDS PAGE followed by western blot with 67466-1-Ig (PSMG3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using PSMG3 Monoclonal antibody (67466-1-Ig)

    WB analysis of A549 using 67466-1-Ig

    A549 cells were subjected to SDS PAGE followed by western blot with 67466-1-Ig (PSMG3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PSMG3 Monoclonal antibody (67466-1-Ig)

    IF Staining of HeLa using 67466-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PSMG3 antibody (67466-1-Ig, Clone: 2H9C3 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

PSMG3 Monoclonal antibody

PSMG3 Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 67466-1-Ig
Clone No.2H9C3
Publications(1)

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

C7orf48, hPAC3, PAC 3, PAC3, PAC-3

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inLNCaP cells, Jurkat cells, K-562 cells, HL-60 cells, A549 cells, Hela cells, HEK-293 cells
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

67466-1-Ig targets PSMG3 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag8384

Product name: Recombinant human PSMG3 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-122 aa of BC004308

Sequence: MEDTPLVISKQKTEVVCGVPTQVVCTAFSSHILVVVTQFGKMGTLVSLEPSSVASDVSKPVLTTKVLLGQDEPLIHVFAKNLVAFVSQEAGNRAVLLAVAVKDKSMEGLKALREVIRVCQVW

相同性解析による交差性が予測される生物種
Full Name proteasome (prosome, macropain) assembly chaperone 3
Calculated molecular weight 13 kDa
Observed molecular weight13 kDa
GenBank accession numberBC004308
Gene Symbol PSMG3
Gene ID (NCBI) 84262
RRIDAB_2882695
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ9BT73
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PSMG3, also named as C7orf48 and PAC3, is a chaperone protein which promotes assembly of the 20S proteasome. It may cooperate with PSMG1-PSMG2 heterodimers to orchestrate the correct assembly of proteasomes.

Protocols

Product Specific Protocols
IF protocol for PSMG3 antibody 67466-1-IgDownload protocol
WB protocol for PSMG3 antibody 67466-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

bioRxiv

PI31 is a positive regulator of 20S immunoproteasome assembly

Authors - Jason Wang
View Article
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