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Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using PSME4 Polyclonal antibody (18799-1-AP)

    WB analysis of HEK-293 using 18799-1-AP (same clone as 18799-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 18799-1-AP (PSME4 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18799-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using PSME4 Polyclonal antibody (18799-1-AP)

    WB analysis of HepG2 using 18799-1-AP (same clone as 18799-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 18799-1-AP (PSME4 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18799-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using PSME4 Polyclonal antibody (18799-1-AP)

    WB analysis of HepG2 using 18799-1-AP (same clone as 18799-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 18799-1-AP (PSME4 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18799-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using PSME4 Polyclonal antibody (18799-1-AP)

    WB analysis of HEK-293 using 18799-1-AP (same clone as 18799-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 18799-1-AP (PSME4 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18799-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HEK-293 cells using PSME4 Polyclonal antibody (18799-1-AP)

    IP experiment of HEK-293 using 18799-1-AP (same clone as 18799-1-PBS)

    IP result of anti-PSME4 (IP:18799-1-AP, 3ug; Detection:18799-1-AP 1:500) with HEK-293 cells lysate 1760ug. This data was developed using the same antibody clone with 18799-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using PSME4 Polyclonal antibody (18799-1-AP)

    IHC staining of human liver cancer using 18799-1-AP (same clone as 18799-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18799-1-AP (PSME4 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18799-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using PSME4 Polyclonal antibody (18799-1-AP)

    IHC staining of human liver cancer using 18799-1-AP (same clone as 18799-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18799-1-AP (PSME4 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18799-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using PSME4 Polyclonal antibody (18799-1-AP)

    IF Staining of HepG2 using 18799-1-AP (same clone as 18799-1-PBS)

    Immunofluorescent analysis of HepG2 cells, using PSME4 antibody 18799-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 18799-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PSME4 Polyclonal antibody (18799-1-AP)

    IF Staining of HeLa using 18799-1-AP (same clone as 18799-1-PBS)

    Immunofluorescent analysis of Hela cells, using PSME4 antibody 18799-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 18799-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using PSME4 Polyclonal antibody (18799-1-AP)

    IF Staining of HepG2 using 18799-1-AP (same clone as 18799-1-PBS)

    Immunofluorescent analysis of HepG2 cells, using PSME4 antibody 18799-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 18799-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

PSME4 Polyclonal antibody, PBS Only

PSME4 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 18799-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

18799-1-PBS targets PSME4 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

Peptide

相同性解析による交差性が予測される生物種
Full Name proteasome (prosome, macropain) activator subunit 4
Calculated molecular weight 211 kDa
Observed molecular weight 140 kDa
GenBank accession numberBC113668
Gene Symbol PSME4
Gene ID (NCBI) 23198
RRIDAB_10598020
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ14997
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

PSME4, like PSME1, 2, and 3, encodes proteasome activator complex subunit 4 also named proteasome activator 200 kDa (PA200). PA200 activates proteasomal cleavage of peptides in an energy-independent manner. After exposure of cells to ionizing radiation, PA200 forms nuclear foci and enhances proteasome activity independent of the stage of cell cycle arrest. PA200 also may be involved in spermatogenesis. The antibody is expected to recognize all the 4 isoforms of PSME4 but appears to mainly detect isoform 2 of 140-kDa.

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