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Validation Data Gallery

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  • Western Blot (WB) analysis of MCF-7 cells using PSME2 Polyclonal antibody (12937-2-AP)

    WB analysis of MCF-7 using 12937-2-AP (same clone as 12937-2-PBS)

    MCF7 cells were subjected to SDS PAGE followed by western blot with 12937-2-AP (PSME2 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12937-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human brain tissue using PSME2 Polyclonal antibody (12937-2-AP)

    WB analysis of human brain using 12937-2-AP (same clone as 12937-2-PBS)

    human brain tissue were subjected to SDS PAGE followed by western blot with 12937-2-AP (PSME2 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12937-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse pancreas tissue using PSME2 Polyclonal antibody (12937-2-AP)

    WB analysis of mouse pancreas using 12937-2-AP (same clone as 12937-2-PBS)

    mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 12937-2-AP (PSME2 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12937-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human kidney tissue using PSME2 Polyclonal antibody (12937-2-AP)

    WB analysis of human kidney using 12937-2-AP (same clone as 12937-2-PBS)

    human kidney tissue were subjected to SDS PAGE followed by western blot with 12937-2-AP (PSME2 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12937-2-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of MCF-7 cells using PSME2 Polyclonal antibody (12937-2-AP)

    IP experiment of MCF-7 using 12937-2-AP (same clone as 12937-2-PBS)

    IP result of anti-PSME2 (IP:12937-2-AP, 4ug; Detection:12937-2-AP 1:500) with MCF-7 cells lysate 2000ug. This data was developed using the same antibody clone with 12937-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using PSME2 Polyclonal antibody (12937-2-AP)

    IHC staining of human kidney using 12937-2-AP (same clone as 12937-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12937-2-AP (PSME2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12937-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using PSME2 Polyclonal antibody (12937-2-AP)

    IHC staining of human kidney using 12937-2-AP (same clone as 12937-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12937-2-AP (PSME2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12937-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using PSME2 Polyclonal antibody (12937-2-AP)

    IF Staining of MCF-7 using 12937-2-AP (same clone as 12937-2-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using PSME2 antibody (12937-2-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). This data was developed using the same antibody clone with 12937-2-PBS in a different storage buffer formulation.

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PSME2 Polyclonal antibody, PBS Only

PSME2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 12937-2-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

REG-beta, REGbeta, REG beta, PA28beta, PA28B

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

12937-2-PBS targets PSME2 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PSME2 fusion protein Ag3986 相同性解析による交差性が予測される生物種
Full Name proteasome (prosome, macropain) activator subunit 2 (PA28 beta)
Calculated molecular weight 239 aa, 27 kDa
Observed molecular weight 27 kDa
GenBank accession numberBC019885
Gene Symbol PSME2
Gene ID (NCBI) 5721
RRIDAB_2300265
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UL46
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

The principal function of the proteasome is targeted degradation of intracellular proteins. Activity of the 20S proteasome is controlled by regulatory complexes that bind to the ends of the cylindrical proteasome. 11S regulator (REG or PA28), is a complex of 28 kDa subunits that is thought to activate proteasomes toward the production of antigenic peptides. Human PSME1 and PSME2 genes encode the two proteasome activators PA28 alpha and beta, respectively, which have been implicated in antigen processing for loading class I MHC molecules. The PA28 activator complex enhances the generation of class I binding peptides by altering the cleavage pattern of the proteasome.