Validation Data Gallery

Filter: AllWBIHCIF/ICCFC (Intra)

WB analysis using 14899-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 14899-1-AP (PSMD4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis of K-562 using 14899-1-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 14899-1-AP (PSMD4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 14899-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 14899-1-AP (PSMD4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of rat spleen using 14899-1-AP
rat spleen tissue were subjected to SDS PAGE followed by western blot with 14899-1-AP (PSMD4 Antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.
IHC staining of human lung cancer using 14899-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14899-1-AP (PSMD4 Antibody) at dilution of 1:200 (under 10x lens).
IHC staining of human lung cancer using 14899-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14899-1-AP (PSMD4 Antibody) at dilution of 1:200 (under 40x lens).
IHC staining of human testis using 14899-1-AP
Immunohistochemical analysis of paraffin-embedded human testis slide using 14899-1-AP (PSMD4 Antibody) at dilution of 1:50.
IHC staining of human testis using 14899-1-AP
Immunohistochemical analysis of paraffin-embedded human testis slide using 14899-1-AP (PSMD4 Antibody) at dilution of 1:50.
IF Staining of HepG2 using 14899-1-AP
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using PSMD4 antibody (14899-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).
FC experiment of HepG2 using 14899-1-AP
1x10^6 HepG2 cells were intracellularly stained with 0.8 ug Anti-Human PSMD4 (14899-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.8 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

Featured Product
KD/KO Validated

PSMD4 Polyclonal antibody

PSMD4 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Cat No. 14899-1-AP

Publications(16)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Synonyms

26S proteasome non-ATPase regulatory subunit 4, AF, ASF, MCB1, Multiubiquitin chain-binding protein

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Overview

Tested Applications
Product Information
Publications (16)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	Jurkat cells, rat spleen tissue, K-562 cells, HeLa cells, NIH/3T3 cells, RAW264.7 cells, Raji cells
Positive IHC detected in	human lung cancer tissue, human testis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells
Positive FC (Intra) detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:5000-1:50000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 3 publications below
WB	See 12 publications below
IHC	See 2 publications below
IF	See 2 publications below

Product Information

14899-1-AP targets PSMD4 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag6691 Product name: Recombinant human PSMD4 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-377 aa of BC002365 Sequence: MVLESTMVCVDNSEYMRNGDFLPTRLQAQQDAVNIVCHSKTRSNPENNVGLITLANDCEVLTTLTPDTGRILSKLHTVQPKGKITFCTGIRVAHLALKHRQGKNHKMRIIAFVGSPVEDNEKDLVKLAKRLKKEKVNVDIINFGEEEVNTEKLTAFVNTLNGKDGTGSHLVTVPPGPSLADALISSPILAGEGGAMLGLGASDFEFGVDPSADPELALALRVSMEEQRQRQEEEARRAAAASAAEAGIATTGTEDSDDALLKMTISQQEFGRTGLPDLSSMTEEEQIAYAMQMSLQGAEFGQAESADIDASSAMDTSEPAKEEDDYDVMQDPEFLQSVLENLPGVDPNNEAIRNAMGSLASQATKDGKKDKKEEDKK 相同性解析による交差性が予測される生物種
Full Name	proteasome (prosome, macropain) 26S subunit, non-ATPase, 4
Calculated molecular weight	41 kDa
Observed molecular weight	50 kDa
GenBank accession number	BC002365
Gene Symbol	PSMD4
Gene ID (NCBI)	5710
RRID	AB_2170627
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P55036
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

PSMD4, also named as MCB1, AF or ASF, belongs to the proteasome subunit S5A family. PSMD4 is a 50-kD subunit of the regulatory complex of the 26S proteasome. It binds and presumably selects ubiquitin-conjugates for destruction. PSMD4 displays selectivity for longer polyubiquitin chains. It modulates intestinal fluid secretion. PSMD4 is exposed on the sperm acrosomal surface, a notion that was further supported by the binding of non-cell permeant, biotinylated proteasomal inhibitor ZL3VS to the sperm acrosome. It is involved in the sperm-ZP penetration during fertilization. (PMID: 20526895) This antibody is specific to PSMD4.

Protocols

Product Specific Protocols
FC protocol for PSMD4 antibody 14899-1-AP	Download protocol
IF protocol for PSMD4 antibody 14899-1-AP	Download protocol
IHC protocol for PSMD4 antibody 14899-1-AP	Download protocol
WB protocol for PSMD4 antibody 14899-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Cancer Res The Ubiquitin-like Protein FAT10 Stabilizes eEF1A1 Expression to Promote Tumor Proliferation in a Complex Manner. Authors - Xiuxia Liu View Article
mouse	WB,IF	Nat Neurosci Loss of nuclear UBE3A causes electrophysiological and behavioral deficits in mice and is associated with Angelman syndrome. Authors - Rossella Avagliano Trezza View Article
human	WB	Nat Commun Molecular basis of SAP05-mediated ubiquitin-independent proteasomal degradation of transcription factors Authors - Xiaojie Yan View Article
human		Pharmacol Res Curcumin modulates β-catenin stabilization via targeting proteasomal deubiquitinating enzyme USP14 Authors - Mengzhen Shen View Article
human	WB	Oncogene The degradation of p53 and its major E3 ligase Mdm2 is differentially dependent on the proteasomal ubiquitin receptor S5a. Authors - Sparks A A View Article
human	WB	Mol Cell Proteomics Proteaphagy in Mammalian Cells Can Function Independent of ATG5/ATG7. Authors - Tatjana Goebel View Article

Validation Data Gallery

WB analysis using 14899-1-AP

WB analysis of K-562 using 14899-1-AP

WB analysis of HeLa using 14899-1-AP

WB analysis of rat spleen using 14899-1-AP

IHC staining of human lung cancer using 14899-1-AP

IHC staining of human lung cancer using 14899-1-AP

IHC staining of human testis using 14899-1-AP

IHC staining of human testis using 14899-1-AP

IF Staining of HepG2 using 14899-1-AP

FC experiment of HepG2 using 14899-1-AP

PSMD4 Polyclonal antibody

PSMD4 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

The Ubiquitin-like Protein FAT10 Stabilizes eEF1A1 Expression to Promote Tumor Proliferation in a Complex Manner.

Loss of nuclear UBE3A causes electrophysiological and behavioral deficits in mice and is associated with Angelman syndrome.

Molecular basis of SAP05-mediated ubiquitin-independent proteasomal degradation of transcription factors

Curcumin modulates β-catenin stabilization via targeting proteasomal deubiquitinating enzyme USP14

The degradation of p53 and its major E3 ligase Mdm2 is differentially dependent on the proteasomal ubiquitin receptor S5a.

Proteaphagy in Mammalian Cells Can Function Independent of ATG5/ATG7.

Able™

Able^™