Validation Data Gallery

Filter: AllWBIPIHC

WB analysis using 12054-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 12054-1-AP (PSMD3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis of A549 using 12054-1-AP
WB result of PSMD3 antibody (12054-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PSMD3 transfected A549 cells.
WB analysis of human brain using 12054-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 12054-1-AP (PSMD3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 12054-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 12054-1-AP (PSMD3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human kidney using 12054-1-AP
human kidney tissue were subjected to SDS PAGE followed by western blot with 12054-1-AP (PSMD3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
IP experiment of mouse kidney using 12054-1-AP
IP result of anti-PSMD3 (IP:12054-1-AP, 4ug; Detection:12054-1-AP 1:500) with mouse kidney tissue lysate 4000ug.
IHC staining of human colon cancer using 12054-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12054-1-AP (PSMD3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human small intestine using 12054-1-AP
Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 12054-1-AP (PSMD3 antibody) at dilution of 1:500 (under 10x lens).
IHC staining of human small intestine using 12054-1-AP
Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 12054-1-AP (PSMD3 antibody) at dilution of 1:500 (under 40x lens).
IHC staining of human breast cancer using 12054-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12054-1-AP (PSMD3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 12054-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12054-1-AP (PSMD3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 12054-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12054-1-AP (PSMD3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Featured Product
KD/KO Validated

PSMD3 Polyclonal antibody

PSMD3 Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 12054-1-AP

Publications(4)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, ELISA

Synonyms

26S proteasome non-ATPase regulatory subunit 3, P58, RPN3, S3, TSTA2

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (4)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	A549 cells, human brain tissue, human kidney tissue, mouse brain tissue, HEK-293 cells, mouse kidney tissue, rat kidney tissue
Positive IP detected in	mouse kidney tissue
Positive IHC detected in	human colon cancer tissue, human breast cancer tissue, human small intestine tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:10000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 4 publications below
IF	See 1 publications below

Product Information

12054-1-AP targets PSMD3 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag2684 Product name: Recombinant human PSMD3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 235-534 aa of BC012302 Sequence: LRTATLRHDADGQATLLNLLLRNYLHYSLYDQAEKLVSKSVFPEQANNNEWARYLYYTGRIKAIQLEYSVARRTMTNALRKAPQHTAVGFKQTVHKLLIVVELLLGEIPDRLQFRQPSLKRSLMPYFLLTQAVRTGNLAKFNQVLDQFGEKFQADGTYTLIIRLRHNVIKTGVRMISLSYSRISLADIAQKLQLDSPEDAEFIVAKAIRDGVIEASINHEKGYVQSKEMIDIYSTREPQLAFHQRISFCLDIHNMSVKAMRFPPKSYNKDLESAEERREREQQDLEFAKEMAEDDDDSFP 相同性解析による交差性が予測される生物種
Full Name	proteasome (prosome, macropain) 26S subunit, non-ATPase, 3
Calculated molecular weight	534 aa, 61 kDa
Observed molecular weight	61 kDa
GenBank accession number	BC012302
Gene Symbol	PSMD3
Gene ID (NCBI)	5709
RRID	AB_2170484
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	O43242
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

PSMD3, encoding proteasome (prosome, macropain) 26S subunit, non-ATPase 3, a member of the 26S proteasome family. PSMD3 is expressed in most of the tissues according to the RNA-Seq database. It has been reported that PSMD3 genotypes are associated with white blood cell (WBC) and neutrophil counts and have interactions with dietary fatty acids and carbohydrates on glucose-related traits. PSMD3 is located at ch17q21 and encodes a protein with a molecular weight of 61 kDa (PMID: 31013812).

Protocols

Product Specific Protocols
IHC protocol for PSMD3 antibody 12054-1-AP	Download protocol
IP protocol for PSMD3 antibody 12054-1-AP	Download protocol
WB protocol for PSMD3 antibody 12054-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Sci Rep Insights into the ubiquitin-proteasome system of human embryonic stem cells. Authors - Isabel Saez View Article
human	WB	Protein Cell DSSylation, a novel protein modification targets proteins induced by oxidative stress, and facilitates their degradation in cells. Authors - Yinghao Zhang View Article
human	WB	Cell Death Dis ALG-2 couples T cell activation and apoptosis by regulating proteasome activity and influencing MCL1 stability. Authors - Tian-Sheng He View Article
human	WB,IF	Cell Mol Life Sci The contribution of proteasomal impairment to autophagy activation by C9orf72 poly-GA aggregates Authors - Mei Pu View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 12054-1-AP

WB analysis of A549 using 12054-1-AP

WB analysis of human brain using 12054-1-AP

WB analysis of mouse brain using 12054-1-AP

WB analysis of human kidney using 12054-1-AP

IP experiment of mouse kidney using 12054-1-AP

IHC staining of human colon cancer using 12054-1-AP

IHC staining of human small intestine using 12054-1-AP