Validation Data Gallery

Filter: AllWBIPIHC

WB analysis using 14748-1-AP
SKOV-3 cells were subjected to SDS PAGE followed by western blot with 14748-1-AP (PSMD2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 14748-1-AP
WB result of PSMD2 antibody (14748-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PSMD2 transfected HepG2 cells.
WB analysis of HeLa using 14748-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 14748-1-AP (PSMD2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of K-562 using 14748-1-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 14748-1-AP (PSMD2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human heart using 14748-1-AP
human heart tissue were subjected to SDS PAGE followed by western blot with 14748-1-AP (PSMD2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse skeletal muscle using 14748-1-AP
mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 14748-1-AP (PSMD2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IP experiment of K-562 using 14748-1-AP
IP result of anti-PSMD2 (IP:14748-1-AP, 4ug; Detection:14748-1-AP 1:1000) with K-562 cells lysate 3320ug.
IHC staining of human breast cancer using 14748-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14748-1-AP (PSMD2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 14748-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14748-1-AP (PSMD2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Featured Product
KD/KO Validated

PSMD2 Polyclonal antibody

PSMD2 Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 14748-1-AP

Publications(23)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF, IP, CoIP, ELISA

Synonyms

26S proteasome non-ATPase regulatory subunit 2, P97, Rpn1, S2, TRAP2

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (23)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	SKOV-3 cells, HeLa cells, K-562 cells, human heart tissue, mouse skeletal muscle tissue, HepG2 cells
Positive IP detected in	K-562 cells
Positive IHC detected in	human breast cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 3 publications below
WB	See 19 publications below
IHC	See 3 publications below
IF	See 4 publications below
IP	See 2 publications below
CoIP	See 3 publications below

Product Information

14748-1-AP targets PSMD2 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, pig
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag6484 Product name: Recombinant human PSMD2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 561-908 aa of BC002368 Sequence: GLGLNHLGKGEAIEAILAALEVVSEPFRSFANTLVDVCAYAGSGNVLKVQQLLHICSEHFDSKEKEEDKDKKEKKDKDKKEAPADMGAHQGVAVLGIALIAMGEEIGAEMALRTFGHLLRYGEPTLRRAVPLALALISVSNPRLNILDTLSKFSHDADPEVSYNSIFAMGMVGSGTNNARLAAMLRQLAQYHAKDPNNLFMVRLAQGLTHLGKGTLTLCPYHSDRQLMSQVAVAGLLTVLVSFLDVRNIILGKSHYVLYGLVAAMQPRMLVTFDEELRPLPVSVRVGQAVDVVGQAGKPKTITGFQTHTTPVLLAHGERAELATEEFLPVTPILEGFVILRKNPNYDL 相同性解析による交差性が予測される生物種
Full Name	proteasome (prosome, macropain) 26S subunit, non-ATPase, 2
Calculated molecular weight	100 kDa
Observed molecular weight	100 kDa
GenBank accession number	BC002368
Gene Symbol	PSMD2
Gene ID (NCBI)	5708
RRID	AB_2170472
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q13200
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Tumor necrosis factor type 1 receptor-associated protein 2 (TRAP2), encoded by PSMD2 gene, is a non-ATPase regulatory subunit of the 26 proteasome which is involved in the ATP-dependent degradation of ubiquitinated proteins. The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. TRAP2 may also participate in the TNF signalling pathway since it interacts with the tumor necrosis factor type 1 receptor.

Protocols

Product Specific Protocols
IHC protocol for PSMD2 antibody 14748-1-AP	Download protocol
IP protocol for PSMD2 antibody 14748-1-AP	Download protocol
WB protocol for PSMD2 antibody 14748-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
		Signal Transduct Target Ther Glucosidase alpha neutral C promotes influenza virus replication by inhibiting proteosome-dependent degradation of hemagglutinin Authors - Xinzhong Liao View Article
human	WB	Cell Cancer vulnerabilities unveiled by genomic loss. Authors - Nijhawan Deepak D View Article
human	WB	Genes Dis The proteasome activator subunit PSME1 promotes HBV replication by inhibiting the degradation of HBV core protein Authors - Yu Liu View Article
mouse,human	WB,CoIP	J Exp Clin Cancer Res AHSA1 is a promising therapeutic target for cellular proliferation and proteasome inhibitor resistance in multiple myeloma. Authors - Chunyan Gu View Article
mouse,human	WB,IHC,IF,CoIP	Cell Death Dis DNAJA4 suppresses epithelial-mesenchymal transition and metastasis in nasopharyngeal carcinoma via PSMD2-mediated MYH9 degradation Authors - Qun Zhang View Article
human		Pharmacol Res Curcumin modulates β-catenin stabilization via targeting proteasomal deubiquitinating enzyme USP14 Authors - Mengzhen Shen View Article

Validation Data Gallery

WB analysis using 14748-1-AP

WB analysis of HepG2 using 14748-1-AP

WB analysis of HeLa using 14748-1-AP

WB analysis of K-562 using 14748-1-AP

WB analysis of human heart using 14748-1-AP

WB analysis of mouse skeletal muscle using 14748-1-AP

IP experiment of K-562 using 14748-1-AP

IHC staining of human breast cancer using 14748-1-AP

IHC staining of human breast cancer using 14748-1-AP

PSMD2 Polyclonal antibody

PSMD2 Polyclonal Antibody for WB, IHC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Glucosidase alpha neutral C promotes influenza virus replication by inhibiting proteosome-dependent degradation of hemagglutinin

Cancer vulnerabilities unveiled by genomic loss.

The proteasome activator subunit PSME1 promotes HBV replication by inhibiting the degradation of HBV core protein

AHSA1 is a promising therapeutic target for cellular proliferation and proteasome inhibitor resistance in multiple myeloma.

DNAJA4 suppresses epithelial-mesenchymal transition and metastasis in nasopharyngeal carcinoma via PSMD2-mediated MYH9 degradation

Curcumin modulates β-catenin stabilization via targeting proteasomal deubiquitinating enzyme USP14

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