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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PSMD13 Monoclonal antibody

PSMD13 Monoclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, rabbit, pig, chicken

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2G10G6

Cat no : 67807-1-Ig

Synonyms

HSPC027, p40.5, PSMD13, Rpn9, S11

Validation Data Gallery

Applications:
  • WB analysis using 67807-1-Ig

    WB analysis using 67807-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67807-1-Ig (PSMD13 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • WB analysis using 67807-1-Ig

    WB analysis using 67807-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67807-1-Ig (PSMD13 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of SMMC-7721 using 67807-1-Ig

    WB analysis of SMMC-7721 using 67807-1-Ig

    SMMC-7721 cells were subjected to SDS PAGE followed by western blot with 67807-1-Ig (PSMD13 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • WB analysis of HuH-7 using 67807-1-Ig

    WB analysis of HuH-7 using 67807-1-Ig

    HuH-7 cells were subjected to SDS PAGE followed by western blot with 67807-1-Ig (PSMD13 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse liver using 67807-1-Ig

    WB analysis of mouse liver using 67807-1-Ig

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 67807-1-Ig (PSMD13 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • WB analysis using 67807-1-Ig

    WB analysis using 67807-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67807-1-Ig (PSMD13 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IHC staining of human pancreas cancer using 67807-1-Ig

    IHC staining of human pancreas cancer using 67807-1-Ig

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67807-1-Ig (PSMD13 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PSMD13 antibody included
  • IHC staining of human pancreas cancer using 67807-1-Ig

    IHC staining of human pancreas cancer using 67807-1-Ig

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67807-1-Ig (PSMD13 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PSMD13 antibody included
  • IHC staining of human lung cancer using 67807-1-Ig

    IHC staining of human lung cancer using 67807-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67807-1-Ig (PSMD13 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PSMD13 antibody included
  • IHC staining of human lung cancer using 67807-1-Ig

    IHC staining of human lung cancer using 67807-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67807-1-Ig (PSMD13 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PSMD13 antibody included
  • IF Staining of HepG2 using 67807-1-Ig

    IF Staining of HepG2 using 67807-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PSMD13 antibody (67807-1-Ig, Clone: 2G10G6 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inHCT 116 cells, SMMC-7721 cells, HepG2 cells, HuH-7 cells, mouse liver tissue, NIH/3T3 cells, HSC-T6 cells, K-562 cells, Jurkat cells, HEK-293 cells, HeLa cells, L02 cells, A549 cells, Calu-3 cells, rat liver tissue, rabbit liver tissue, pig liver, chicken liver
Positive IHC detected inhuman pancreas cancer tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy PSMD13 Ready-To-Use IHC Kit. PSMD13 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:2000-1:8000
Immunofluorescence (IF)IF : 1:400-1:1600
Sample-dependent, check data in validation data gallery

Product Information

67807-1-Ig targets PSMD13 in WB, IF, IHC, ELISA applications and shows reactivity with Human, mouse, rat, rabbit, pig, chicken samples.

Tested Reactivity Human, mouse, rat, rabbit, pig, chicken
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PSMD13 fusion protein Ag7857 相同性解析による交差性が予測される生物種
Full Name proteasome (prosome, macropain) 26S subunit, non-ATPase, 13
Calculated molecular weight 43 kDa
Observed molecular weight43 kDa
GenBank accession numberBC001100
Gene symbol PSMD13
Gene ID (NCBI) 5719
RRIDAB_2918570
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for PSMD13 antibody 67807-1-IgDownload protocol
IHC protocol for PSMD13 antibody 67807-1-IgDownload protocol
IF protocol for PSMD13 antibody 67807-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols