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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PSMD10/Gankyrin Polyclonal antibody (12342-2-AP)

    WB analysis using 12342-2-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12342-2-AP (PSMD10/Gankyrin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse skin tissue using PSMD10/Gankyrin Polyclonal antibody (12342-2-AP)

    IHC staining of mouse skin using 12342-2-AP

    Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 12342-2-AP (PSMD10/Gankyrin antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse skin tissue using PSMD10/Gankyrin Polyclonal antibody (12342-2-AP)

    IHC staining of mouse skin using 12342-2-AP

    Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 12342-2-AP (PSMD10/Gankyrin antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human urothelial carcinoma tissue using PSMD10/Gankyrin Polyclonal antibody (12342-2-AP)

    IHC staining of human urothelial carcinoma using 12342-2-AP

    Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 12342-2-AP (PSMD10/Gankyrin antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human urothelial carcinoma tissue using PSMD10/Gankyrin Polyclonal antibody (12342-2-AP)

    IHC staining of human urothelial carcinoma using 12342-2-AP

    Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 12342-2-AP (PSMD10/Gankyrin antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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PSMD10/Gankyrin Polyclonal antibody

PSMD10/Gankyrin Polyclonal Antibody for WB, IHC, ELISA
Cat No. 12342-2-AP
Publications(4)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, ELISA

Synonyms

PSMD10, p28(GANK), p28, Gankyrin, 26S proteasome non-ATPase regulatory subunit 10

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inA549 cells, HEK-293 cells, HeLa cells, K-562 cells, PC-3 cells
Positive IHC detected inmouse skin tissue, human urothelial carcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 4 publications below

Product Information

12342-2-AP targets PSMD10/Gankyrin in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PSMD10/Gankyrin fusion protein Ag2994 相同性解析による交差性が予測される生物種
Full Name proteasome (prosome, macropain) 26S subunit, non-ATPase, 10
Calculated molecular weight 226 aa, 24 kDa
Observed molecular weight 24-28 kDa
GenBank accession numberBC011960
Gene Symbol PSMD10
Gene ID (NCBI) 5716
RRIDAB_2172809
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO75832
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for PSMD10/Gankyrin antibody 12342-2-APDownload protocol
IHC protocol for PSMD10/Gankyrin antibody 12342-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

J Biomed Sci

Znf179 E3 ligase-mediated TDP-43 polyubiquitination is involved in TDP-43- ubiquitinated inclusions (UBI) (+)-related neurodegenerative pathology.

Authors - Yi-Chao Lee
View Article
humanWB

Appl Biochem Biotechnol

miR-188-5p and Host MALAT1 Regulate RBE Cell Migration, Invasion, and Apoptosis via Up-regulating PSMD10 in Cholangiocarcinoma

Authors - Zhaoqiang Yin
View Article
humanWB

Research (Wash D C)

SERBP1 Promotes Stress Granule Clearance by Regulating 26S Proteasome Activity and G3BP1 Ubiquitination and Protects Male Germ Cells from Thermostimuli Damage

Authors - Fengli Wang
View Article
human,mouseWB

Elife

A cleaved METTL3 potentiates the METTL3-WTAP interaction and breast cancer progression

Authors - Chaojun Yan
View Article