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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PSMD1 Recombinant monoclonal antibody (83521-2-RR)

    WB analysis using 83521-2-RR (same clone as 83521-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83521-2-RR (PSMD1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83521-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A431 cells using PSMD1 Recombinant monoclonal antibody (83521-2-RR)

    WB analysis of A431 using 83521-2-RR (same clone as 83521-2-PBS)

    WB result of PSMD1 antibody (83521-2-RR; 1:1500; incubated at room temperature for 1.5 hours) with sh-Control and sh-PSMD1 transfected A431 cells. This data was developed using the same antibody clone with 83521-2-PBS in a different storage buffer formulation.

  • Sandwich ELISA sample test of MP00519-3

    Sandwich ELISA sample test of MP00519-3

    Mouse stomach tissue lysate and Jurkat cell lysate were measured. The human PSMD1 concentration of detected samples was determined to be 11,510.8 pg/mL in mouse stomach lysate (based on a 4.0 mg/mL extract load) and 17,515.2 pg/mL in Jurkat cell lysate (based on a 3.4 mg/mL extract load).

  • Sandwich ELISA standard curve of MP00519-3

    Sandwich ELISA standard curve of MP00519-3

    Sandwich ELISA standard curve of MP00519-3, Human PSMD1 Recombinant Matched Antibody Pair - PBS only. 83521-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag12009. 83521-5-PBS was HRP conjugated as the detection antibody. Range: 78.1-5000 pg/mL

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using PSMD1 Recombinant monoclonal antibody (83521-2-RR)

    IHC staining of human intrahepatic cholangiocarcinoma using 83521-2-RR (same clone as 83521-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 83521-2-RR (PSMD1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83521-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using PSMD1 Recombinant monoclonal antibody (83521-2-RR)

    IHC staining of human intrahepatic cholangiocarcinoma using 83521-2-RR (same clone as 83521-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 83521-2-RR (PSMD1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83521-2-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of MCF-7 cells using PSMD1 Recombinant monoclonal antibody (83521-2-RR)

    FC experiment of MCF-7 using 83521-2-RR (same clone as 83521-2-PBS)

    1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug PSMD1 Recombinant antibody (83521-2-RR, Clone:240458B3) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83521-2-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83521-2-PBS

    Affinity and Kinetic Characterization of 83521-2-PBS

    Biolayer interferometry (BLl) kinetic assays of 83521-2-PBS against Human PSMD1 were performed. The affinity constant is below 1 pM.

  • Affinity and Kinetic Characterization of 83521-2-RR

    Affinity and Kinetic Characterization of 83521-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83521-2-RR against Human PSMD1 were performed. The affinity constant is below 1 pM.

  • Featured Product
  • KD/KO Validated

PSMD1 Recombinant monoclonal antibody, PBS Only (Capture)

PSMD1 Uni-rAb® Recombinant Antibody for WB, IHC, FC (Intra), Sandwich ELISA, Indirect ELISA
Cat No. 83521-2-PBS
Clone No.240458B3

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, FC (Intra), Sandwich ELISA, Indirect ELISA

Synonyms

240458B3, 26S proteasome non-ATPase regulatory subunit 1, PSMD 1

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83521-2-PBS targets PSMD1 as part of a matched antibody pair:

MP00519-3: 83521-2-PBS capture and 83521-5-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag12009

Product name: Recombinant human PSMD1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 606-953 aa of BC094720

Sequence: AVSDVNDDVRRAAVESLGFILFRTPEQCPSVVSLLSESYNPHVRYGAAMALGICCAGTGNKEAINLLEPMTNDPVNYVRQGALIASALIMIQQTEITCPKVNQFRQLYSKVINDKHDDVMAKFGAILAQGILDAGGHNVTISLQSRTGHTHMPSVVGVLVFTQFWFWFPLSHFLSLAYTPTCVIGLNKDLKMPKVQYKSNCKPSTFAYPAPLEVPKEKEKEKVSTAVLSITAKAKKKEKEKEKKEEEKMEVDEAEKKEEKEKKKEPEPNFQLLDNPARVMPAQLKVLTMPETCRYQPFKPLSIGGIIILKDTSEDIEELVEPVAAHGPKIEEEEQEPEPPEPFEYIDD

相同性解析による交差性が予測される生物種
Full Name proteasome (prosome, macropain) 26S subunit, non-ATPase, 1
Calculated molecular weight 953 aa, 106 kDa
Observed molecular weight106 kDa
GenBank accession numberBC094720
Gene Symbol PSMD1
Gene ID (NCBI) 5707
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ99460
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

PSMD1 is a component of the 26S proteasome and contributes to its function regulation. PSMD1 performs an important role in the regulation of numerous cellular processes through the degradation of proteins targeted for breakdown through ubiquitin. PSMD1 has been found to be closely related to a variety of cancers such as lung adenocarcinoma and breast cancer.

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