Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse brain tissue, human brain tissue, human testis tissue, rat liver tissue, mouse liver tissue, rat brain tissue |
| Positive IHC detected in | human liver cancer tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 3 publications below |
| IHC | See 1 publications below |
Product Information
15839-1-AP targets PSMC6 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag8589 Product name: Recombinant human PSMC6 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-389 aa of BC005390 Sequence: MADPRDKALQDYRKKLLEHKEIDGRLKELREQLKELTKQYEKSENDLKALQSVGQIVGEVLKQLTEEKFIVKATNGPRYVVGCRRQLDKSKLKPGTRVALDMTTLTIMRYLPREVDPLVYNMSHEDPGNVSYSEIGGLSEQIRELREVIELPLTNPELFQRVGIIPPKGCLLYGPPGTGKTLLARAVASQLDCNFLKVVSSSIVDKYIGESARLIREMFNYARDHQPCIIFMDEIDAIGGRRFSEGTSADREIQRTLMELLNQMDGFDTLHRVKMIMATNRPDTLDPALLRPGRLDRKIHIDLPNEQARLDILKIHAGPITKHGEIDYEAIVKLSDGFNGADLRNVCTEAGMFAIRADHDFVVQEDFMKAVRKVADSKKLESKLDYKPV 相同性解析による交差性が予測される生物種 |
| Full Name | proteasome (prosome, macropain) 26S subunit, ATPase, 6 |
| Calculated molecular weight | 389 aa, 44 kDa |
| Observed molecular weight | 42 kDa |
| GenBank accession number | BC005390 |
| Gene Symbol | PSMC6 |
| Gene ID (NCBI) | 5706 |
| RRID | AB_2237797 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P62333 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
PSMC6(proteasome (prosome, macropain) 26S subunit, ATPase, 6) is also named as CADP44, MGC12520, P44, SUG2, p42 and belongs to the AAA ATPase family. It is a component of the 26S protease, which is involved in the ATP-dependent degradation of ubiquitinated proteins. The regulatory (or ATPase) complex confers ATP dependency and substrate specificity to the 26S complex. The gene encodes a 44 kDa PSMC6.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for PSMC6 antibody 15839-1-AP | Download protocol |
| WB protocol for PSMC6 antibody 15839-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Biomed Sci Znf179 E3 ligase-mediated TDP-43 polyubiquitination is involved in TDP-43- ubiquitinated inclusions (UBI) (+)-related neurodegenerative pathology. | ||
Cardiovasc Drugs Ther Quercetin Prevents In Vivo and In Vitro Myocardial Hypertrophy Through the Proteasome-GSK-3 Pathway. | ||
Acta Pharmacol Sin Zonisamide attenuates pressure overload-induced myocardial hypertrophy in mice through proteasome inhibition | ||
Cell Rep Med Integrative proteomic profiling of tumor and plasma extracellular vesicles identifies a diagnostic biomarker panel for colorectal cancer |
