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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PSMC1 Recombinant antibody (86237-1-RR)

    WB analysis using 86237-1-RR (same clone as 86237-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86237-1-RR (PSMC1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86237-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using PSMC1 Recombinant antibody (86237-1-RR)

    IHC staining of mouse cerebellum using 86237-1-RR (same clone as 86237-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 86237-1-RR (PSMC1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86237-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of Jurkat cells using PSMC1 Recombinant antibody (86237-1-RR)

    IF Staining of Jurkat using 86237-1-RR (same clone as 86237-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using PSMC1 antibody (86237-1-RR, Clone: 251014B9 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 86237-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 86237-1-RR

    Affinity and Kinetic Characterization of 86237-1-RR

    Biolayer interferometry (BLl) kinetic assays of 86237-1-RR against Human PSMC1 were performed. The affinity constant is 0.402 nM.

PSMC1 Recombinant monoclonal antibody, PBS Only

PSMC1 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 86237-1-PBS
Clone No.251014B9

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

26S proteasome AAA-ATPase subunit RPT2, 26S proteasome regulatory subunit 4, P26S4

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

86237-1-PBS targets PSMC1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag1677

Product name: Recombinant human PSMC1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 77-440 aa of BC016368

Sequence: EFIRNQEQMKPLEEKQEEERSKVDDLRGTPMSVGTLEEIIDDNRAIVSTSVGSEHYVSILSFVDKDLLEPGCSVLLNHKVHAVIGVLMDDTDPLVTVMKVEKAPQETYADIGGLDNQIQEIKESVELPLTHPEYYEEMGIKPPKGVILYGPPGTGKTLLAKAVANQTSATFLRVVGSELIQKYLGDGPKLVRELFRVAEEHAPSIVFIDEIDAIGTKRYDSNSGGEREIQRTMLELLNQLDGFDSRGDVKVIMATNRIETLDPALIRPGRIDRKIEFPLPDEKTKKRIFQIHTSRMTLADDVTLDDLIMAKDDLSGADIKAICTEAGLMALRERRMKVTNEDFKKSKENVLYKKQEGTPEGLYL

相同性解析による交差性が予測される生物種
Full Name proteasome (prosome, macropain) 26S subunit, ATPase, 1
Calculated molecular weight 49 kDa
Observed molecular weight52-60 kDa
GenBank accession numberBC016368
Gene Symbol PSMC1
Gene ID (NCBI) 5700
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP62191
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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