• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using PSMB9 Monoclonal antibody (67748-1-Ig)

    WB analysis using 67748-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67748-1-Ig (PSMB9 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • Western Blot (WB) analysis of rat spleen tissue using PSMB9 Monoclonal antibody (67748-1-Ig)

    WB analysis of rat spleen using 67748-1-Ig

    rat spleen tissue were subjected to SDS PAGE followed by western blot with 67748-1-Ig (PSMB9 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using PSMB9 Monoclonal antibody (67748-1-Ig)

    IHC staining of human colon cancer using 67748-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67748-1-Ig (PSMB9 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using PSMB9 Monoclonal antibody (67748-1-Ig)

    IHC staining of human colon cancer using 67748-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67748-1-Ig (PSMB9 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human spleen tissue using PSMB9 Monoclonal antibody (67748-1-Ig)

    IHC staining of human spleen using 67748-1-Ig

    Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 67748-1-Ig (PSMB9 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

PSMB9 Monoclonal antibody

PSMB9 Monoclonal Antibody for WB, IHC, ELISA
Cat No. 67748-1-Ig
Clone No.1C7C8

Host / Isotype

Mouse / IgG2a

Reactivity

Human, rat

Applications

WB, IHC, ELISA

Synonyms

beta1i, LMP2, Low molecular mass protein 2, Macropain chain 7, Proteasome chain 7, Proteasome subunit beta 1i, Proteasome subunit beta type 9, PSMB6i, PSMB9, RING12

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inRaji cells, rat spleen tissue, Ramos cells, Daudi cells, HL-60 cells, THP-1 cells
Positive IHC detected inhuman colon cancer tissue, human spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:300-1:1200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67748-1-Ig targets PSMB9 in WB, IHC, ELISA applications and shows reactivity with Human, rat samples.

Tested Reactivity Human, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag6802

Product name: Recombinant human PSMB9 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-219 aa of BC065513

Sequence: MLRAGAPTGDLPRAGEVHTGTTIMAVEFDGGVVMGSDSRVSAGEAVVNRVFDKLSPLHERIYCALSGSAADAQAVADMAAYQLELHGIELEEPPLVLAAANVVRNISYKYREDLSAHLMVAGWDQREGGQVYGTLGGMLTRQPFAIGGSGSTFIYGYVDAAYKPGMSPEECRRFTTDAIALAMSRDGSSGGVIYLVTITAAGVDHRVILGNELPKFYDE

相同性解析による交差性が予測される生物種
Full Name proteasome (prosome, macropain) subunit, beta type, 9 (large multifunctional peptidase 2)
Calculated molecular weight 23 kDa
Observed molecular weight20-23 kDa
GenBank accession numberBC065513
Gene Symbol PSMB9
Gene ID (NCBI) 5698
RRIDAB_2918517
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP28065
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IHC protocol for PSMB9 antibody 67748-1-IgDownload protocol
WB protocol for PSMB9 antibody 67748-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
||
New chat Able
正在加载，请稍候...